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Current Microbiology

, Volume 24, Issue 3, pp 171–177 | Cite as

Amphotericin B synergy testing by the FCST

  • R. Scott Pore
Article

Abstract

The flow cytofluorometric susceptibility test (FCST) was incorporated into two in vitro synergy assays of amphotericin B-drug combinations. The FCST checkerboard assay and the FCST concentration-effect assay were developed to detect subtle modulation of amphotericin B fungicidal effects onCandida albicans andCryptococcus neoformans. Amphotericin B × cyclosporine A was a synergistic fungicidal combination against bothC. albicans andC. neoformans. Amphotericin B×gentamicin and amphotericin B×ketoconazole were synergistic combinations againstC. neoformans. In all cases tested, the synergy was effective when 0.50–0.62 μg amphotericin B/ml was used with>-0.50 μg of the other drug/ml. The fungicidal effect of ≥1.00 μg amphotericin B/ml overwhelmed the synergistic effects. A number of other drug combinations were additive, autonomous, or antagonistic.

Keywords

Cyclosporine Synergistic Effect Gentamicin Amphotericin Ketoconazole 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc 1992

Authors and Affiliations

  • R. Scott Pore
    • 1
  1. 1.Department of Microbiology and ImmunologyWest Virginia University School of MedicineMorgantownUSA

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