Current Microbiology

, Volume 19, Issue 3, pp 153–161 | Cite as

(1–3)-β-Glucan synthesis ofNeurospora crassa

  • Evelyn Jabri
  • David R. Quigley
  • Marjorie Alders
  • Maria Hrmova
  • Cathy S. Taft
  • Patricia Phelps
  • Claude P. Selitrennikoff


(1–3)-β-d-Glucan synthase activity ofNeurospora crassa was localized to the plasma membrane by autoradiography of colloidal gold-labeled plasma membranes. The active site of glucan synthase for substrate hydrolysis was determined to be cytoplasmic facing. However, glucan synthase activity present in intact protoplasts was partially sensitive to Novozym 234 and to glutaraldehyde treatments, suggestive that enzyme activity is transmembrane. Enzyme activity also directed the formation of microfibrils in vitro. Taken together, these and previous results support the following scheme for glucan synthesis: 1. The sequential addition of glucose residues from UDP-glucose to glucan chains occurs on the cytoplasmically facing portion of glucan synthase. 2. As each glucan chain is synthesized, it is extruded to the extracytoplasmic side of the plasma membrane. 3. As each chain is extruded, it forms interchain hydrogen bonds with adjacent chains, resulting in glucan microfibril assembly.


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Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • Evelyn Jabri
    • 1
  • David R. Quigley
    • 1
  • Marjorie Alders
    • 1
  • Maria Hrmova
    • 1
  • Cathy S. Taft
    • 1
  • Patricia Phelps
    • 1
  • Claude P. Selitrennikoff
    • 1
  1. 1.Department of Cellular and Structural BiologyUniversity of Colorado Health Sciences CenterDenverUSA

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