Advertisement

Current Microbiology

, Volume 19, Issue 1, pp 39–43 | Cite as

Oxygen tolerance of anaerobic bacteria isolated from human feces

  • Tullio Brusa
  • Enrica Canzi
  • Novella Pacini
  • Raffaella Zanchi
  • Annamaria Ferrari
Article

Abstract

The large bowel intestinal flora of mammals is made up mostly of O2-intolerant anaerobic microorganisms which are irreversibly damaged by brief exposure to air. The aim of our work was to investigate the effect of atmospheric O2 on human intestinal anaerobic microorganisms. Thirty O2-intolerant bacterial strains that reached 100% mortality after 120 min of air exposure were isolated. Ten of these strains were tested for their atmospheric O2 sensitivity as a function of air exposure time; all tested microorganisms showed a similar mortality trend on exposure to air. In fact, 50% of cells survive, on the average, after 4–5 min of atmospheric O2; this percentage decreases to 3–5% after only 20 min, and after 40 min only one cell in a thousand survives; all strains reached 100% mortality in a time range of 100–120 min. The strains examined were identified as belonging to the generaEubacterium, Peptostreptococcus, andCoprococcus.

Keywords

Oxygen Exposure Time Bacterial Strain Time Range Large Bowel 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Aranki A, Freiter R (1972) Use of anaerobic glove boxes for the cultivation of strictly anaerobic bacteria. Am J Clin Nutr 25:1329–1334PubMedGoogle Scholar
  2. 2.
    Aranki A, Syed SA, Kennedy EB, Freiter R (1969) Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Appl Microbiol 17:568–576PubMedGoogle Scholar
  3. 3.
    Brusa T, Ferrari A (1986) Studi atti a migliorare i livelli di anossia durante le manipolazioni microbiologiche in cabina anaerobica e modifiche strutturali dell'apparecchiatura. Ann Microbiol Enzimol 36:85–90Google Scholar
  4. 4.
    Carlsson J, Nyberg G, Wrethen J (1978) Hydrogen peroxide and superoxide radical formation in anaerobic broth media exposed to atmospheric oxygen. Appl Environ Microbiol 36:223–229PubMedGoogle Scholar
  5. 5.
    Drasar BS (1967) Cultivation of anaerobic intestinal bacteria. J Pathol Bacteriol 94:417–427PubMedGoogle Scholar
  6. 6.
    Ferrari A, Pacini N, Canzi E, Bruno F (1980) Presence of oxygen intolerant microorganisms in primary bile acid 7-dehydroxylating mouse intestinal microflora. Curr Microbiol 4:257–260Google Scholar
  7. 7.
    Fesce A (1973) La flora microbica intestinale. Folia Vet Latina 3:635–652Google Scholar
  8. 8.
    Fesce A, Ceccarelli A, Poli G, Balsari A, Giovannini R (1977) Metodo per l'isolamento e lo studio di batteri anaerobici ossigeno intolleranti. Arch Vet Ital 28:1–9Google Scholar
  9. 9.
    Finegold SM, Sutter VL, Mathisen GE (1983) Normal indigenous intestinal flora. In: Hentges DS (ed) Human intestinal flora in health and disease. New York: Academic Press, pp 3–31Google Scholar
  10. 10.
    Fredette V, Planté C, Roy A (1967) Numerical data concerning the sensitivity of anaerobic bacteria to oxygen. J Bacteriol 93:2012–2017Google Scholar
  11. 11.
    Fridovich I (1983) Superoxide radical an endogenous toxicant. Annu Rev Pharmacol Toxicol 23:239–257PubMedGoogle Scholar
  12. 12.
    Gregory EM, Moore WEC, Holdeman LV (1978) Superoxide dismutase in anaerobes: survey. Appl Environ Microbiol 35:988–991PubMedGoogle Scholar
  13. 13.
    Gustafsson BE (1982) The physiological importance of the colonic microflora. Scand J Gastroenterol 17/77:117–131Google Scholar
  14. 14.
    Hassan HM, Fridovich I (1980) Superoxide dismutase: detoxication of a free radical. In: Jakoby WB (ed) Enzymatic basis of detoxication, vol 1. New York: Academic Press, pp 311–332Google Scholar
  15. 15.
    Holdeman LV, Moore WEC (1973) Anaerobic laboratory manual, 4th edn. Blaksburg, VA: Virginia Polytechnic Institute and State University Anaerobe Laboratory.Google Scholar
  16. 16.
    Hungate RE (1950) The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev 14:1–49PubMedGoogle Scholar
  17. 17.
    Kikuchi HE, Suzuki T (1986) Quantitative method for measurement of aerotolerance of bacteria and its application to oral indigenous anaerobes. Appl Environ Microbiol 52:971–973PubMedGoogle Scholar
  18. 18.
    Loesche WJ (1969) Oxygen sensitivity of various anaerobic bacteria. Appl Microbiol 18:723–727PubMedGoogle Scholar
  19. 19.
    Mevissen-Verhage EAE, De Vos NM, Harmsen van Amerongen WCM, Marcelis JH (1982) A selective medium for the detection and enumeration of clostridia in human facces. Antonie van Leeuwenhoek 48:205–206Google Scholar
  20. 20.
    Moore WHC (1966) Techniques for routine culture of fastidious anaerobes. Int J Syst Bacteriol 16:173–190Google Scholar
  21. 21.
    Morris JG (1976) Oxygen and the obligate anaerobe. J Appl Bacteriol 40:229–244PubMedGoogle Scholar
  22. 22.
    Onderdonk AB, Johnston J, Mayhew JW, Gorback SL (1976) Effect of dissolved oxygen on Eh onBacteroides fragilis during continuous culture. Appl Environ Microbiol 31:168–172PubMedGoogle Scholar
  23. 23.
    Patel GB, Roth LA, Agnew BJ (1984) Death rates of obligate anaerobes exposed to oxygen and the effect of media prereduction on cell viability. Can J Microbiol 30:228–235Google Scholar
  24. 24.
    Rolfe RO, Hentges DJ, Barrett TT, Campbell BJ (1977) Oxygen tolerance of human intestinal anaerobes. Am J Clin Nutr 30:1762–1769PubMedGoogle Scholar
  25. 25.
    Rolfe RO, Hentges DJ, Campbell BJ, Barrett TT (1978) Factors related to the oxygen tolerance of anaerobic bacteria. Appl Environ Microbiol 36:306–313PubMedGoogle Scholar
  26. 26.
    Samah OA, Wimpenny JWT (1982) Some effects of oxygen on the physiology ofSelenomonas ruminantium WPL 151/1 growth in continuous culture. J Gen Microbiol 128:335–360PubMedGoogle Scholar
  27. 27.
    Schwartz AC (1973) Anaerobiosis and oxygen consumption of some strains ofPropionibacterium and a modified method for comparing the oxygen sensitivity of various anaerobes. Z Allg Mikrobiol 13:681–691PubMedGoogle Scholar
  28. 28.
    Sneath PHA, Mair NS, Sharpe ME, Holt JG (1986) Bergey's manual of systematic bacteriology, 1st ed. Baltimore, London, Los Angeles: Williams & WilkinsGoogle Scholar
  29. 29.
    Tally FP, Stewart PR, Sutter VR, Rosemblatt JE (1975) Oxygen tolerance of fresh clinical anaerobic bacteria. J Clin Microbiol 1:161–164PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • Tullio Brusa
    • 1
  • Enrica Canzi
    • 1
  • Novella Pacini
    • 1
  • Raffaella Zanchi
    • 1
  • Annamaria Ferrari
    • 1
  1. 1.Department of Food Science and Microbiology, Section of Agricultural, Food and Ecological MicrobiologyUniversity of MilanMilanItaly

Personalised recommendations