Current Microbiology

, Volume 8, Issue 3, pp 183–186 | Cite as

Thioacetamide as a source of hydrogen sulfide for colony growth of purple sulfur bacteria

  • Roar L. Irgens


Thioacetamide (TAA), CH3CSNH2, is an unstable sulfur compound which upon addition of acid decomposes into acetic acid, ammonia, and hydrogen sulfide (H2S). This characteristic may be taken advantage of when doing plate counts or isolation streaks of phototrophic purple sulfur bacteria. We have performed plate counts and isolation streaks of these bacteria in Gas-Pak anaerobic jars (BBL) supplemented with a test tube containing 0.05 g or 0.10 g TAA dissolved in 1.0 ml of 0.2 N or 0.5 N HCl. It may be demonstrated in a Warburg respirometer that the gas is released over a period of at least 1 week. Colony growth may be observed in 5–10 days. One advantage of this technique is that sodium sulfide (Na2S·9H2O) need not be added to the agar medium, which, therefore, may be prepared and stored for future use. This technique has been used successfully for the isolation ofAmoebobacter, Chromatium, Ectothiorhodospira, Lamprocystis, Thiocapsa, andThiocystis.


Sulfur Acetic Ammonia Sulfide Agar 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Clayton, R. K., W. R. Sistrom. 1978. The photosynthetic bacteria. New York, London: Plenum Press.Google Scholar
  2. 2.
    Irgens, R. L. 1977.Meniscus, a new genus of aerotolerant, gas-vacuolated bacteria. International Journal of Systematic Bacteriology27:38–43.Google Scholar
  3. 3.
    Pfennig, N. 1967. Photosynthetic bacteria. Annual Review of Microbiology21:285–324.PubMedGoogle Scholar
  4. 4.
    van Niel, C. B. 1971. Techniques for the enrichment, isolation, and maintenance of the photosynthetic bacteria. Methods in Enzymology23:3–28.Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Roar L. Irgens
    • 1
  1. 1.Department of BiologySouthwest Missouri State UniversitySpringfieldUSA

Personalised recommendations