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Current Microbiology

, Volume 14, Issue 6, pp 311–317 | Cite as

Cloning and characterization of genes coding for ribosomal RNA inCampylobacter jejuni

  • Ayoub Raschtchian
  • Mark A. Abbott
  • Marilee Shaffer
Article

Abstract

The DNA fragments coding for ribosomal RNA inCampylobacter jejuni have been cloned from a genomic library ofC. jejuni constructed inEscherichia coli. Clones carrying DNA Sequences for rRNA were identified by hybridization of 5′-end-labeled rRNA fromC. jejuni to colony blots of transformants from this gene library. Cloned DNA sequences homologous to each of 5S, 16S, and 23S rRNA were idenfified by hybridization of labeled plasmid DNA to Northern blots of rRNA. The gene coding for 23S rRNA was found to be located on a 5.5kb HindIII fragment, while the 5S and 16S rRNA genes were on HindIII fragments of 1.65 and 1.7 kb, respecitively. The DNA fragment containing the 16S rRNA gene was characterized by restriction endonuclease mapping, and the location of the 16S rRNA gene on this fragment was determined by hybridization of 5′-end-labeled rRNA to restriction fragments and also by DNA sequence determination. It appears that the major portion of the coding region for 16S rRNA is located on the 1.7-kb HindIII fragment, while a small portion is carried on an adjacent HindIII fragment of 7.5 kb. Cloned rRNA genes fromC. jejuni were used to study the organization of the rDNA inC. jejuni and other members of the genùsCampylobacter.

Keywords

Restriction Endonuclease Northern Blot Restriction Fragment Major Portion Genomic Library 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc 1987

Authors and Affiliations

  • Ayoub Raschtchian
    • 1
  • Mark A. Abbott
    • 1
  • Marilee Shaffer
    • 1
  1. 1.Integrated Genetics, Inc.FraminghamUSA

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