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Current Microbiology

, Volume 13, Issue 1, pp 11–16 | Cite as

Acholeplasmas and similar nonsterol-requiring mollicutes from insects: Missing link in microbial ecology

  • Truman B. Clark
  • Joseph G. Tully
  • David L. Rose
  • Roberta Henegar
  • Robert F. Whitcomb
Article

Abstract

Wall-less prokaryotes from guts of five insect species (a corn root maggot [Plecia sp.: Diptera: Bibionidae, strain PS-1], a syrphid fly [Diptera: Syrphidae, strain YJS], two tabanid flies [Tabanus catenatus, strain TAC, andChrysops discalis, strain DF-2], and a vespid wasp [Monobia quadridens, strain MQ-3]) were characterized. The strains grew at 23°–32°C in conventional mycoplasma media containing 10% (vol/vol) serum, or in serum-free mycoplasma medium with or without fatty-acid-Tween-80 supplements. No helical forms were noted in dark-field microscopy, and electron micrographs of thin sections of the strains showed a single membrane. Two strains (DF and MQ-3) cross reacted with antiserum againstAcholeplasma florum. Although the other three strains did not react with antisera to establishedAcholeplasma orMycoplasma species, they were otherwise characteristic of acholeplasmas. This study, the first to demonstrate acholeplasmas from Arthropoda, in conjunction with previous isolations from plant surfaces, suggests that insects may constitute an important reservoir for acholeplasmas.

Keywords

Corn Thin Section Insect Species Plant Surface Important Reservoir 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Truman B. Clark
    • 1
  • Joseph G. Tully
    • 2
  • David L. Rose
    • 2
  • Roberta Henegar
    • 1
  • Robert F. Whitcomb
    • 1
  1. 1.Insect Pathology Laboratory, Plant Protection Institute, Agricultural Research ServiceUS Department of AgricultureBeltsvilleUSA
  2. 2.Mycoplasma Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious DiseasesFrederick Cancer Research FacilityFrederickUSA

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