Evidence for lysogeny and viral resistance in the cyanobacteriumPhormidium uncinatum
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Abstract
Cyanophage LPP-1-induced lysogens and a resistant mutant of the cyanobacteriumPhormidium uncinatum were isolated and characterized. In lysogens, spontaneous lysis occurred and increased with the growth of the host cyanobacterium. The virus-liberating property of the lysogens was not lost with the viricidal concentration of EDTA, and the titer obtained was >3±103 PFU ml−1. Heat and UV treatment of lysogens failed to induce lysis, but mitomycin C induced lysis by fivefold. The adsorption rate of the virus on the lysogens was slower than on the sensitive parent host. The resistant mutant lost its virus-adsorbing property.
Keywords
EDTA Mitomycin Resistant Mutant Adsorption Rate Viral Resistance
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