Current Microbiology

, Volume 14, Issue 1, pp 13–17 | Cite as

Expression of chloramphenicol resistance specified by plasmid pHY416 hosted inCorynebacterium glutamicum

  • Margaret L. Britz
  • Geoffrey R. Best


Chloramphenicol (Cm)-resistant colonies ofCorynebacterium glutamicum strain AS019 hosting plasmid pHY416 occurred at a frequency of 4×10−7 when this strain, which normally expresses kanamycin (Km) resistance only, was placed under positive selection. These isolates produced a chloramphenicol acetyltransferase (CAT) and were tenfold more resistant to Cm than the parent strain. Resistance was lost in the absence of Cm selection but could be reselected from Km-resistant clones by reapplying Cm selection. Restriction endonuclease analyses of plasmids extracted from Cm-sensitive and-resistant strains indicated that expression of CAT activity corresponded to a loss of 0.9 kb of DNA from plasmid pHY416.


Restriction Endonuclease Kanamycin Chloramphenicol Parent Strain Restriction Endonuclease Analysis 
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Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • Margaret L. Britz
    • 1
  • Geoffrey R. Best
    • 2
  1. 1.Industrial Microbiology Unit, Division of Chemical and Wood TechnologyCSIROClaytonAustralia
  2. 2.Department of Applied BiologyRMITMelbourneAustralia

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