Immunogenetics

, Volume 11, Issue 1, pp 7–19 | Cite as

Secretion of either of a pair of immunoglobulins, IgM or IgX, in somatic hybrid cells derived by fusion of a B-cell lymphoma cell line carrying both immunoglobulin isotypes

  • Nobuhiko Tada
  • Ulrich Hämmerling
Original Investigations
Received:
Revised:

Abstract

The I.29 cell line is a nonsecreting B-cell leukemia which bears two different immunoglobulin isotypes on its surface, IgM and IgX. The I.29 cells were hybridized with nonsecreting myeloma cells giving rise to dozens of immunoglobulin secreting hybridomas. These fall into three groups differing in the class of immunoglobulin they secrete. Cells of the first group secrete pentameric IgM (μ, κ), those of the second group secrete an unknown immunoglobulin, IgX, which may constitute an allotype of IgA, and those of the third group produce light chains only. The two complete immunoglobulins, IgM and IgX, have the same idiotype, as revealed by serological cross-reactivity of an exhaustively absorbed rabbit anti-idiotype serum.

The molecular sizes of the heavy chains of the secreted IgM and IgX are slightly smaller than theμ andδ chains, respectively, which are derived from the surface of normal B cells as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Keywords

Lymphoma Myeloma Light Chain Myeloma Cell Somatic Hybrid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this paper

Ig

immunoglobulin

NMS

normal mouse serum

NaDodSO4

sodium dodecyl sulfate

PAGE

polyacrylamide gel electrophoresis

2ME

2-mercaptoethanol

BPB

bromophenol blue

NP40

nonidet P40

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Nobuhiko Tada
    • 1
  • Ulrich Hämmerling
    • 1
  1. 1.Memorial Sloan-Kettering Cancer CenterNew York

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