Current Microbiology

, Volume 5, Issue 2, pp 109–114

Polynucleotide sequence relatedness among motileAeromonas species

  • Michel Y. Popoff
  • Colette Coynault
  • Martine Kiredjian
  • Muguette Lemelin
Article
  • 46 Downloads

Abstract

Relatedness among 55 motileAeromonas strains was assessed by determining the extent of reassociation in heterologous DNA preparations. S1 nuclease and diethylaminoethyl-cellulose filters were used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of related nucleotide sequences. The motile aeromonads would presently consist of three species:A. hydrophila (type strain ATCC 7966),A. caviae (type strain ATCC 15468), andA. sobria (type strain CIP 7433). These three species are clearly separated on the basis of both biochemical characteristics and similarities in DNA. Each of these three species contains more than one hybridization group: three groups inA. hydrophila; two groups inA. caviae; and at least two groups inA. sobria. DNA hybridization groups are biochemically similar within each species. When additional data is available, these separate DNA hybridization groups may merit designation. Any decision to delineate species in terms of DNA hybridization groups must await a phenotypic basis for their separation.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Brenner, D. J., Fanning, G. R., Johnson, K. E., Citarella, R. V., Falkow, S. 1969. Polynucleotide sequence relationships among members of theEnterobacteriaceae. Journal of Bacteriology98:637–650.PubMedGoogle Scholar
  2. 2.
    Brenner, D. J., Fanning, G. R., Skerman, F. J., Falkow, S. 1972. Polynucleotide sequence divergence among strains ofEscherichia coli and closely related organisms. Journal of Bacteriology109:963–965.Google Scholar
  3. 3.
    Brenner, D. J., Fanning, G. R., Steigerwalt, A. G., Ørskov, I., Ørskov, F. 1972. Polynucleotide sequence relatedness among three groups of pathogenicEscherichia coli strains. Infection and Immunity6:308–315.PubMedGoogle Scholar
  4. 4.
    Brenner, D. J., Fanning, G. R., Steigerwalt, A. G. 1972. Deoxyribonucleic acid relatedness among species ofErwinia and betweenErwinia species and other enterobacteria. Journal of Bacteriology110:12–17.PubMedGoogle Scholar
  5. 5.
    Brenner, D. J., Steigerwalt, A. G., Falcão, D. P., Weaver, R. F., Fanning, G. R. 1976. Characterization ofYersinia enterocolitica andYersinia pseudotuberculosis by deoxyribonucleic acid hybridization and by biochemical reactions. International Journal of Systematic Bacteriology26:180–194.Google Scholar
  6. 6.
    Cooley, W. W., Lohnes, P. R. 1971. Multivariate data analysis. New York: Wiley.Google Scholar
  7. 7.
    Crosa, J. H., Brenner, D. J., Falkow, S. 1973. Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. Journal of Bacteriology115:904–911.PubMedGoogle Scholar
  8. 8.
    Grimont, P. A. D., Popoff, M. Y. 1980. Use of principal component analysis in interpretation of deoxyribonucleic acid relatedness. Current Microbiology4:337–342.Google Scholar
  9. 9.
    Grimont, P. A. D., Popoff, M. Y., Grimont, F., Coynault, C., Lemelin, M. 1980. Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Current Microbiology4:325–330.Google Scholar
  10. 10.
    Hope, K. 1968. Methods of multivariate analysis. London: University of London Press.Google Scholar
  11. 11.
    Kendall, M. 1975. Multivariate analysis. London: Griffin.Google Scholar
  12. 12.
    McCarthy, D. H. 1975. The bacteriology and taxonomy ofAeromonas liquefaciens. Technical Report Series, no. 2. Weymouth, Dorset: Fish Diseases Laboratory.Google Scholar
  13. 13.
    MacInnes, J. I., Trust, T. J., Crosa, J. H. 1979. Deoxyribonucleic acid relationships among members of the genusAeromonas. Canadian Journal of Microbiology25:579–586.PubMedGoogle Scholar
  14. 14.
    McConaughy, B. L., McCarthy, B. J. 1970. Related base sequences in the DNA of simple and complex organisms. VI. The extent of base sequence divergence among the DNAs of various rodents. Biochemical Genetics4:425–446.PubMedGoogle Scholar
  15. 15.
    Owen, R. J., Hill, L. R., Lapage, S. P. 1969. Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers7:503–516.PubMedGoogle Scholar
  16. 16.
    Popoff, M., Coynault, C. 1980. Use of DEAE-cellulose filters in the Sl nuclease method for bacterial deoxyribonucleic acid hybridization. Annales de Microbiologie131A:151–155.PubMedGoogle Scholar
  17. 17.
    Popoff, M., Véron, M. 1976. A taxonomic study of theAeromonas hydrophila-Aeromonas punctata group. Journal of General Microbiology94:11–22.PubMedGoogle Scholar
  18. 18.
    Schubert, R. H. W. 1974. Genus II.Aeromonas, pp. 345–348. In: Buchanan, R. E., Gibbons, N. E. (eds.), Bergey's manual of determinative bacteriology, 8th ed. Baltimore: Williams & Wilkins.Google Scholar
  19. 19.
    Shaw, D. H., Hodder, H. J. 1978. Lipopolysaccharides of the motile aeromonads; core oligosaccharide analysis as an aid to taxonomic classification. Canadian Journal of Microbiology24:864–868.PubMedGoogle Scholar
  20. 20.
    Skerman, V. B. D., McGowan, V., Sneath, P. H. A. 1980. Approved lists of bacterial names. International Journal of Systematic Bacteriology30:225–420.Google Scholar
  21. 21.
    Steigerwalt, A. G., Fanning, G. R., Fife-Asbury, M. A., Brenner, D. J. 1976. DNA relatedness among species ofEnterobacter andSerratia. Canadian Journal of Microbiology22:121–137.PubMedGoogle Scholar
  22. 22.
    Stoleru, G. H., Le Minor, L., Lheritier, A. M. 1976. Polynucleotide sequence divergence among strains ofSalmonella sub-genus IV and closely related organisms. Annales de Microbiologie127A:477–486.Google Scholar
  23. 23.
    Vogt, V. N. 1973. Purification and further properties of single-strand-specific nuclease fromAspergillus oryzae. European Journal of Biochemistry33:192–200.PubMedGoogle Scholar
  24. 24.
    Wetmur, J. G., Davidson, N. 1968. Kinetics of renaturation of DNA. Journal of Molecular Biology31:349–370.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1981

Authors and Affiliations

  • Michel Y. Popoff
    • 1
  • Colette Coynault
    • 1
  • Martine Kiredjian
    • 1
  • Muguette Lemelin
    • 1
  1. 1.Service des EntérobacteriesINSERM Unité 199, Institut PasteurParis Cedex 15France

Personalised recommendations