Current Microbiology

, Volume 11, Issue 5, pp 269–274 | Cite as

Immunogold detection of chromatophore antigens on the surface ofRhodopseudomonas sphaeroides spheroplasts

  • Mary Lynne Perille Collins
  • Susan T. Remson
Article
  • 22 Downloads

Abstract

Antibody was obtained from rabbits immunized with chromatophores isolated fromRhodopseudomonas sphaeroides. Antichromatophore antibody was adsorbed with membranes prepared from nonphotosynthetic aerobically grown cells to remove cross-reacting antibody in order to obtain an antibody preparation specific for antigens unique to the chromatophore membrane. This adsorbed antibody was coupled to colloidal gold particles and used to label chromatophore antigens on the surface of spheroplasts of phototrophically grown cells. Aerobically grown cells were induced to form chromatophore membrane by reduction of oxygen tension. Chromatophore-specific antigens were recognized by crossed immunoelectrophoretic analysis of membranes prepared from these induced cells and by immunogold labeling of spheroplasts prepared from these cells.

Keywords

Oxygen Gold Oxygen Tension Gold Particle Colloidal Gold 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Mary Lynne Perille Collins
    • 1
  • Susan T. Remson
    • 1
  1. 1.Department of Biological SciencesUniversity of Wisconsin-MilwaukeeMilwaukeeUSA

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