Advertisement

Current Microbiology

, Volume 6, Issue 4, pp 229–231 | Cite as

Vigorous denitrification by a heterotrophic nitrifier of the genusAlcaligenes

  • Domenic Castignetti
  • Thomas C. Hollocher
Article

Abstract

A heterotrophic nitrifyingAlcaligenes sp., previously isolated from soil and shown to be very active in the aerobic oxidation of pyruvic oxime (and hydroxylamine) to nitrite, is now shown to be quite active as a denitrifier. The bacterium synthesized nitrite, nitrous oxide, and nitrogen gas from nitrate when grown anaerobically and could individually reduce nitrate, nitrite, nitric oxide, and nitrous oxide to nitrogen gas when these nitrogen-oxides were added to dense cell suspensions. No evidence was obtained for the release of nitric oxide during reduction of nitrate and nitrite. The specific rates of reduction of the nitrogen-oxide were similar to those of well-known laboratory strains of denitrifying bacteria. The induction of an entire set of denitrifying enzymes at normal levels in a heterotrophic nitrifier is novel. The nitrification-denitrification capability ofAlcaligenes sp. may confer certain advantages to this and analogous organisms in the environment.

Keywords

Oxide Enzyme Nitrate Nitrite Cell Suspension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Alexander, M., Marshall, K. C., Hirsch, P. 1960. Autotrophy and heterotrophy in nitrification. Proceedings, 7th International Conference of Soil Science, Madison, Wisconsin,2:586–591.Google Scholar
  2. 2.
    Amarger, N., Alexander, M. 1968. Nitrite formation from hydroxylamine and oximes byPseudomonas aeruginosa. Journal of Bacteriology95:1651–1657.PubMedGoogle Scholar
  3. 3.
    Castignetti, D., Gunner, H. B. 1980. Sequential nitrification by anAlcaligenes sp. andNitrobacter agilis. Canadian Journal of Microbiology26:1114–1119.PubMedGoogle Scholar
  4. 4.
    Castignetti, D., Gunner, H. B. 1981. Nitrite and nitrate formation by anAlcaligenes sp. Current Microbiology5:379–384.Google Scholar
  5. 5.
    Chang, J. P., Morris, J. G. 1962. Studies on the utilization of nitrate byMicrococcus denitrificans. Journal of General Microbiology29:301–310.PubMedGoogle Scholar
  6. 6.
    Focht, D. D., Verstraete, W. 1977. Biochemical ecology of nitrification and denitrification. Advances in Microbial Ecology1:135–214.Google Scholar
  7. 7.
    Garber, E. A. E., Hollocher, T. C. 1981.15N Tracer studies on the role of NO in denitrification. Journal of Biological Chemistry256:5459–5465.PubMedGoogle Scholar
  8. 8.
    Herbert, D., Phipps, P. J., Strange, R. E. 1971. Chemical analysis of microbial cells, pp. 242–252. In: Norris, J. R., Ribbons, D. S. (eds.), Methods in microbiology, vol. 5B. London, New York: Academic Press.Google Scholar
  9. 9.
    Hollocher, T. C., Garber, E., Cooper, A. J. L., Reiman, R. E. 1980.13N,15N Isotope and kinetic evidence against hyponitrite as an intermediate in denitrification. Journal of Biological Chemistry255:5027–5030.PubMedGoogle Scholar
  10. 10.
    Kristjansson, J. K., Hollocher, T. C. 1979. Substrate binding site for nitrate reductase ofEscherichia coli is on the inner aspect of the membrane. Journal of Bacteriology137:1227–1233.PubMedGoogle Scholar
  11. 11.
    Nason, A. 1962. Symposium on metabolism of inorganic compounds. II. Enzymatic pathways of nitrate, nitrite, and hydroxylamine metabolism. Bacteriological Reviews26:16–41.PubMedGoogle Scholar
  12. 12.
    Obaton, M., Amarger, A., Alexander, M. 1968. Heterotrophic nitrification byPseudomonas aeruginosa. Archiv für Mikrobiologie63:122–132.Google Scholar
  13. 13.
    Payne, W. J. 1973. Reduction of nitrogen oxides by microorganisms. Bacterological Reviews37:409–452.Google Scholar
  14. 14.
    Pichinoty, F., D'Orano, L. 1961. Sur la mechanisme de l'inhibition par l'oxygene de la denitrification bacterienne. Biochimica et Biophysica Acta52:386–389.PubMedGoogle Scholar
  15. 15.
    Shimizu, T., Furuki, T., Waki, J., Ichikawa, K. 1978. Metabolic characteristics of denitrification byParacoccus denitrificans. Journal of Fermentation Technology56:207–213.Google Scholar
  16. 16.
    Sidransky, E., Walter, B., Hollocher, T. C. 1978. Studies on the differential inhibition by azide on the nitrite/nitrous oxide level of denitrification. Applied and Environmental Microbiology35:247–250.PubMedGoogle Scholar
  17. 17.
    St. John, R. T., Hollocher, T. C. 1977. Nitrogen-15 tracer studies on the pathway of denitrification inPseudomonas aeruginosa. Journal of Biological Chemistry252:212–218.PubMedGoogle Scholar
  18. 18.
    Van'T Riet, J., Stouthamer, A. H., Planta, R. J. 1968. Regulation of nitrate assimilation and nitrate respiration inAerobacter aerogenes. Journal of Bacteriology96:1455–1464.PubMedGoogle Scholar
  19. 19.
    Verstraete, W. 1975. Heterotrophic nitrification in soils and aqueous media. Translated from Izvestiya Akademii Nauk SSSR, Seriya Biologicheskaya4:541–558.Google Scholar
  20. 20.
    Walker, N. 1978. On the diversity of nitrifiers in nature, pp. 346–347. In: Schlessinger, D. (ed.), Microbiology-1978. Washington, D.C.: American Society for Microbiology.Google Scholar
  21. 21.
    Walters, B., Sidransky, E., Kristjansson, J. K., Hollocher, T. C. 1978. Inhibition of denitrification by uncouplers of oxidative phosphorylation. Biochemistry17:3039–3042.PubMedGoogle Scholar
  22. 22.
    Yoshida, T., Alexander, M. 1970. Nitrous oxide formation byNitrosomonas europea and heterotrophic micro-organisms. Proceedings of the Soil Science Society of America34:880–882.Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1981

Authors and Affiliations

  • Domenic Castignetti
    • 1
  • Thomas C. Hollocher
    • 1
  1. 1.Graduate Department of BiochemistryBrandeis UniversityWalthamUSA

Personalised recommendations