Current Microbiology

, Volume 7, Issue 5, pp 285–290 | Cite as

Wall-less prokaryotes from fall flowers in central United States and Maryland

  • Robert F. Whitcomb
  • Joseph G. Tully
  • David L. Rose
  • Edward B. Stephens
  • Alexis Smith
  • Randolph E. McCoy
  • Michael F. Barile


Four spiroplasma strains and eleven isolates tentatively identified as acholeplasmas were obtained from fall flowers in Colorado, Nebraska, Illinois, and Maryland. Although the acholeplasma isolates were heterogeneous, all showed antigenic sharing with a group of unnamed organisms (L1 and related strains) isolated in othe studies from flowers in Florida. The W20 and W24 isolates from Nebraska were partially related to the L1 group by DNA-DNA homology and polyacrylamide gel electrophoresis (PAGE) analyses. A Colorado spiroplasma (W13) was identifed as a new strain of group IV complex. Three spiroplasma strains from flowers in Maryland “old fields” represent a new serovar with closest affinity to subgroup I-4 and to the LB12 and N525 serovars of group I. Widespread occurrence of acholeplasmas on flowers in this study, and on plant surfaces in general, suggests that, like spiroplasmas they probably will be found to reside in arthropods.


United States Electrophoresis Polyacrylamide Related Strain Plant Surface 
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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Robert F. Whitcomb
    • 1
  • Joseph G. Tully
    • 2
  • David L. Rose
    • 2
  • Edward B. Stephens
    • 3
  • Alexis Smith
    • 4
  • Randolph E. McCoy
    • 4
  • Michael F. Barile
    • 3
  1. 1.Insect Pathology Laboratory, Plant Protection Institute, Agricultural Research, Science and Education AdministrationU.S. Department of Agriculture, Building 465. Beltsville Agricultural Research Center-EastBelltsvilleUSA
  2. 2.Mycoplasma Section, Laboratory of Molecular MicribiologyNational Institute of Allergy and Infectious Disearses, Building 550, Frederick Cancer Research CenterFrederickUSA
  3. 3.Mycoplasma Branch, Division of Bacterial Products, Bureau of BiologicsFood and Drug AdministrationBethesdaUSA
  4. 4.Ariculture Research CenterUniversity of FloridaFort LauderdaleUSA

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