Acta diabetologia latina

, Volume 9, Issue 1, pp 924–957 | Cite as

Cyclophosphamide inhibition of insulin antibody production, insulin resistance and experimental immunodiabetes

  • Lidka Korčáková
  • Milan Titlbach
  • Karel Nouza
Original Contributions


Hyperglycemia, increase of insulin tolerance and striking changes in B- and A-cells of the islets of Langerhans occurred in guinea pigs producing precipitating insulin antibodies after immunization with insulin in adjuvant. Treatment with cyclophosphamide inhibited the production of insulin antibodies while the blood sugar and insulin tolerance remained on the level of nonimmunized control animals. Also the islets of Langerhans were less severely injured than those of animals not treated with cyclophosphamide.


Cyclophosphamide treatment Guinea pig Experimental diabetes Immunodiabetes Immunosuppression Insulin resistance Islets of Langerhans Precipitating insulin antibodies Ultrastructure 


Iperglicemia, aumento della tolleranza all'insulina e cospicue modificazioni a carico delle cellule B ed A delle isole di Langerhans sono stati osservati, dopo immunizzazione con insulina in adiuvante, in cavie produttrici di anticorpi anti-insulina precipitanti. Il trattamento con ciclofosfamide inibiva la produzione di anticorpi anti-insulina, mentre la glicemia e la tolleranza insulinica rimanevano agli stessi livelli riscontrabili negli animali di controllo non immunizzati. Anche le isole di Langerhans apparivano meno danneggiate rispetto a quelle degli animali non trattati con ciclofosfamide.


Hyperglycémie, amélioration de la tolérance à l'insuline et modifications considérables à niveau des cellules B et A des ilots de Langerhans ont fait l'objet d'une observation, après immunisation par insuline et adjuvant, en cobayes produisant des anticorps anti-insuline précipitants. Le traitement par le cyclophosphamide inhibait la production d'anticorps anti-insuline, alors que la glycémie et la tolérance à l'insuline restaient aux mêmes niveaux qu'on pouvait vérifier chez les animaux de contrôle non immunisés. Même les îlots de Langerhans semblaient avoir subi des dommages moins importants par rapport à ceux des animaux qui n'avaient pas été traités par le cyclophosphamide.


En covayos productores de anticuerpos antinsulina precipitantes, tras inmunización con insulina y adyuvante, se ha podido observar: hiperglicemia, aumento de la tolerancia a la insulina y conspícuas modificaciones a cargo de las células A y B de las islas de Langerhans. El tratamiento con ciclofosfamida inhibía la producción de anticuerpos antinsulina, mientras la glucemia y la tolerancia insulínica permanecían en los mismos niveles que podían observarse en los animales de control no inmunizados. Las islas de Langerhans aparecían menos dañadas respecto a las de los animales no tratados con ciclofosfamida.


Hyperglykämie, erhöhte Insulintoleranz und auffällige Veränderungen der A- und B-Zellen der Langerhansschen Inseln wurden bei Meerschweinchen beobachtet, welche infolge Immunisierung mit Insulin in Adjuvans Insulin-präzipitierende Antikörper bilden. Cyclophosphamid-Behandlung verhindert die Bildung von Insulin-Antikörpern, während Blutzucker und Insulintoleranz auf dem gleichen Niveau bleiben wie bei nicht immunisierten Kontrolltieren. Auch die Langerhansschen Inseln sind weniger schwer geschädigt als bei nicht mit Cyclophosphamid behandelten immunisierten Tieren.


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Copyright information

© Casa Editrice 1972

Authors and Affiliations

  • Lidka Korčáková
    • 1
    • 2
    • 3
  • Milan Titlbach
    • 1
    • 2
  • Karel Nouza
    • 1
    • 2
  1. 1.Czechoslovak Academy of ScienceLaboratory for Ultrastructure Research of Cells and TissuesCzechoslovakia
  2. 2.Institute of Experimental Biology and GeneticsCzechoslovakia
  3. 3.Laboratoř Elektronové MikroscopiePraha 2Czechoslovakia

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