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Immunogenetics

, Volume 1, Issue 1, pp 457–468 | Cite as

Genetic analysis of anH-2 mutant, B6.C-H-2 ba , using cell-mediated lympholysis: T- and B-cell dictionaries for histocompatibility determinants are different

  • M. Nabholz
  • H. Young
  • T. Meo
  • V. Miggiano
  • A. Rijnbeek
  • D. C. Shreffler
Article

Abstract

B6.C-H-2 ba [H (z1)] is a mutant derived from C57BL/6. The two strains mutually reject their skingrafts and are incompatible in the mixed leucocyte reaction (MLR) and in cell-mediated lympholysis (CML) assays. They are serologically indistinguishable.

This report shows that H(z1) carries a new, privateK end CML specificity clearly distinguishable from that of B6 by a third party strain, HTG. Antisera directed against the private H-2K specificity of B6 present on H(z1) cells) can block CML between the two strains in either direction. The new CML specificities of H(z1) cross-react with (public) CML specificities controlled by bothK andD regions of other unrelated haplotypes. The results suggest that H(z1) carries a mutation in theH-2K locus itself or in a closely linked gene, the product of which is also physically associated with the H-2K molecule corresponding to the cis-configuration of the alleles in both loci.

These findings indicate that T- and B-cell dictionaries for histocompatibility determinants are different.

Keywords

Genetic Analysis andD Region Mixed Leucocyte Mixed Leucocyte Reaction Leucocyte Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1975

Authors and Affiliations

  • M. Nabholz
    • 1
  • H. Young
    • 1
  • T. Meo
    • 1
  • V. Miggiano
    • 1
  • A. Rijnbeek
    • 1
  • D. C. Shreffler
    • 2
  1. 1.Basel Institute for ImmunologyBaselSwitzerland
  2. 2.Department of Human Genetics, Medical SchoolThe University of MichiganAnn Arbor

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