Immunogenetics

, Volume 1, Issue 1, pp 457–468

Genetic analysis of anH-2 mutant, B6.C-H-2ba, using cell-mediated lympholysis: T- and B-cell dictionaries for histocompatibility determinants are different

  • M. Nabholz
  • H. Young
  • T. Meo
  • V. Miggiano
  • A. Rijnbeek
  • D. C. Shreffler
Article

Abstract

B6.C-H-2ba [H (z1)] is a mutant derived from C57BL/6. The two strains mutually reject their skingrafts and are incompatible in the mixed leucocyte reaction (MLR) and in cell-mediated lympholysis (CML) assays. They are serologically indistinguishable.

This report shows that H(z1) carries a new, privateK end CML specificity clearly distinguishable from that of B6 by a third party strain, HTG. Antisera directed against the private H-2K specificity of B6 present on H(z1) cells) can block CML between the two strains in either direction. The new CML specificities of H(z1) cross-react with (public) CML specificities controlled by bothK andD regions of other unrelated haplotypes. The results suggest that H(z1) carries a mutation in theH-2K locus itself or in a closely linked gene, the product of which is also physically associated with the H-2K molecule corresponding to the cis-configuration of the alleles in both loci.

These findings indicate that T- and B-cell dictionaries for histocompatibility determinants are different.

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Copyright information

© Springer-Verlag New York Inc. 1975

Authors and Affiliations

  • M. Nabholz
    • 1
  • H. Young
    • 1
  • T. Meo
    • 1
  • V. Miggiano
    • 1
  • A. Rijnbeek
    • 1
  • D. C. Shreffler
    • 2
  1. 1.Basel Institute for ImmunologyBaselSwitzerland
  2. 2.Department of Human Genetics, Medical SchoolThe University of MichiganAnn Arbor

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