Immunogenetics

, Volume 6, Issue 1, pp 425–432 | Cite as

HLA-B8 phenotype associated with an increased mixed leukocyte reaction

  • David Osoba
  • Judy Falk
Original Investigations
Received:
Revised:

Abstract

Peripheral blood leukocytes from normal volunteers and from patients with untreated Hodgkin's diseases have been assayed for the magnitude of their responses to pools of selected stimulating cells in a quantitative one-way mixed leukocyte reaction (MLR). Responses by leukocytes from individuals withHLA-B8 segregated above the median response regardless of whether the donors were healthy or were patients with Hodgkin's disease. These results suggest the presence of a gene (or genes) in the responding cells that has an effect on the magnitude of the MLR. This gene is in nonrandom association with the gene coding forB8 at theB region of theHLA complex. The observed linkage disequilibrium ofB8 with a gene controlling the proliferative response to alloantigens may be the basis for the increased frequency ofB8 in long-term survivors in carcinoma of the breast and Hodgkin's disease, and for the association ofB8 with several autoaggressive diseases.

Keywords

Carcinoma Linkage Disequilibrium Proliferative Response Normal Volunteer Peripheral Blood Leukocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this paper are

MLR

mixed leukocyte reaction

SPI, SPII, and SPIII

selected pools of stimulating cells

cpm

counts per minute

This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bodmer, W.F.: Evolutionary significance of the HL-A systems.Nature 237:139–145, 1972PubMedGoogle Scholar
  2. Dausset, J., Degros, L., and Hors, J.: The association of the HL-A antigens with diseases.Clin. Immunol. Immunopathol. 5:127–149, 1974Google Scholar
  3. Doherty, P.C., Blanden, R.U., and Zinkernagel, R.N.: Specificity of virus-immune effector T cells for H-2D compatible interactions: implications for H-antigen diversity.Transplant. Rev. 29:89–124, 1976PubMedGoogle Scholar
  4. Eijsvoogel, V.P., van Rood, J.J., Toit, D.E., and Schellekens, P.T.S.: Position of a locus determining mixed lymphocyte reaction distinct from the known HL-A loci.Eur. J. Immunol. 2:413–418, 1972PubMedGoogle Scholar
  5. Eijsvoogel, V.P., duBois, M.J.G.J., Melief, C.J.M., deGroot-Kooy, M.L., Koning, C., van Rood, J.J., van Leeuwen, A., Toit, E.D., and Schellekens, P.T.A.: Position of a locus determining mixed lymphocyte reaction (MLR) distinct from the known HL-A loci, and its relation to cell-mediated lympholysis (CML).In J. Dausset and J. Colombani (eds.):Histocompatibility Testing, 1972; pp. 501–508, Munksgaard, Copenhagen, 1973Google Scholar
  6. Falk, J. and Osoba, D.: HL-A antigens and survival in Hodgkin's disease.Lancet 2:1118–1120, 1971PubMedGoogle Scholar
  7. Falk, J.A. and Osoba, D.: The association of the human histocompatibility system with Hodgkin's disease.J. Immunogenetics 1:53–61, 1974Google Scholar
  8. Falk, J. and Osoba, D.: The HLA system and survival in malignant disease: Hodgkin's disease and carcinoma of the breast.In G.P. Murphy (ed.):HLA and Malignancy; pp. 205–216, Alan R. Liss, Inc., New York, 1977Google Scholar
  9. Mittal, K.K., Mickey, M.R., Singal, D.P., and Terasaki, P.I.: Serotyping for homotransplantation. XVIII. Refinement of microdroplet lymphocyte cytotoxicity test.Transplantation 6:913–927, 1968PubMedGoogle Scholar
  10. Osoba, D. and Falk, J.: The mixed-leukocyte reaction in man: effect of pools of stimulating cells selected on the basis of crossreacting HL-A specificities.Cell. Immunol. 10:117–135, 1974PubMedGoogle Scholar
  11. Osoba, D., Falk, J., Dajko, M., and Richardson, J.: A simple freezing and storage method to preserve the stimulating function of leukocytes in the mixed leukocyte reaction.Tissue Antigens 5:226–233, 1975PubMedGoogle Scholar
  12. Svejgaard, A., Platz, P., Ryder, L.P., Nielsen, L.S., and Thomsen, M.: HL-A and disease associations-a survey.Transplant. Rev. 22:3–43, 1975PubMedGoogle Scholar
  13. Vladutiu, A.O. and Rose, N.R.: HL-A antigens: association with disease.Immunogenet. 1:305–328, 1974Google Scholar
  14. Yunis, E.J. and Amos, D.B.: Three closely linked genetic systems relevant to transplantation.Proc. Natl. Acad. Sci. U.S.A. 68:3031–3035, 1971PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1978

Authors and Affiliations

  • David Osoba
    • 1
    • 3
  • Judy Falk
    • 2
    • 3
  1. 1.Department of MedicineUniversity of Toronto, the Princess Margaret Hospital in The Ontario Cancer InstituteTorontoCanada
  2. 2.Department of PathologyToronto Western HospitalTorontoCanada
  3. 3.Department of SurgeryToronto Western HospitalTorontoCanada

Personalised recommendations