, Volume 10, Issue 1–4, pp 273–281 | Cite as

Bone-marrow-cell grafts involving theH-2D andH-2L mutant haplotypes

  • Michael Bennett
  • Roger W. Melvold
  • Henry I. Kohn
Original Investigations


TwoH-2 d mutants,H-2 dm2 (H-2L loss mutation) andH-2dm1 (gainplus-loss mutation involving bothH-2L andH-2D) were evaluated for any change in the immunogenicity of marrow stem cells. Grafts of 2 or 4 × 106 BALB/c(C) or BALB/c-H-2dm2 (C-H-2 dm 2) marrow cells were accepted by lethally irradiated B10.D2(H-2 d ) recipients and were rejected by irradiated B10(H-2 b ) recipients. Moreover, both (B6 × C)F1 and (B6 × C-H-2 dm 2)F1 mice, as irradiated recipients, resisted the growth of parental-strain B6(H-2 b ) marrow cells but accepted grafts from C or C-H-2 dm 2 parental-strain donors. Thus, theH-2 mutation involvingH-2L but notH-2D did not affect the expression ofH-2 d -associated Hemopoietic or Hybrid(Hh) antigens of marrow stem cells. Grafts of 2 to 8 × 106 B10.D2 or B10.D2-H-2 dm 1 marrow cells were rejected by B10.BR(H-2 k ) and B6 hosts and were accepted by B10.D2 hosts. However, B10.D2-H-2 dm 1 marrow cells grew to a much greater extent than B10.D2 cells in irradiated (B6 × B10.D2)F1 or (B6 × B10.D2-H-2 dm 1)F1 host mice. Therefore, theH-2 dm 1 mutation has altered the expression of Hh antigens at least quantitatively, resulting in a relative loss of “hybrid resistance” with the retention of Hh determinants recognized by allogeneic recipient mice which are notH-2 d . Since the Hh determinants of B10.D2 marrow cells have been mapped 16 cM to the right ofH-2, this mutation atH-2D/H-2L may have affected a regulatory gene.


Stem Cell Regulatory Gene Marrow Cell Recipient Mouse Relative Loss 
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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Michael Bennett
    • 1
  • Roger W. Melvold
    • 2
    • 3
  • Henry I. Kohn
    • 3
  1. 1.Department of PathologyBoston University School of MedicineBoston
  2. 2.Section of Medical Oncology, Departments of Medicine and Microbiology-Immunology and the Cancer CenterNorthwestern University Medical SchoolChicago
  3. 3.Department of Radiation Therapy, Harvard Medical School and Shields Warren Radiation LaboratoryNew England Deaconess HospitalBoston

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