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Immunogenetics

, Volume 10, Issue 1–4, pp 247–260 | Cite as

Monoclonal antibodies identifying a novel T-Cell antigen and Ia antigens of human lymphocytes

  • John A. Hansen
  • Paul J. Martin
  • Robert C. Nowinski
Original Investigations

Abstract

We describe here two new monoclonal antibodies that react with surface antigens of human lymphocytes. Antibody 7.2 identified a determinant on the framework region of the human Ia antigen. It was cytotoxic for all cultured B-cell lines, normal B cells, and monocytes. The antibody was not cytotoxic for normal T cells or for established T leukemic cell lines. In immune precipitation assays, the 7.2 antibody reacted with a bimolecular complex of two chains that resolved in polyacrylamide gels as polypeptides with molecular weights of 29000 and 34000 daltons. These precipitation results were analogous to those achieved with a rabbit antiserum prepared against human Ia antigens. Antibody 9.3 identified a determinant on the framework region of a T-cell antigen. It was cytotoxic for 50–80% of peripheral T cells and for 20–50% of thymocytes. It was not cytotoxic for cultured B-cell lines, normal B cells, or monocytes. In immune precipitation assays, the 9.3 antibody reacted with a single polypeptide with a molecular weight of 44000 daltons. Due to the expression of this antigen on a limited subpopulation of human T cells, we have designated the antigen HuLyt-1.

Keywords

Molecular Weight Monoclonal Antibody Polypeptide Polyacrylamide Leukemic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • John A. Hansen
    • 1
    • 2
  • Paul J. Martin
    • 1
    • 2
  • Robert C. Nowinski
    • 1
    • 2
  1. 1.The Histocompatibility LaboratoryPuget Sound Blood Center, Fred Hutchinson Cancer Research CenterUSA
  2. 2.Division of Medical Oncology, Department of MedicineUniversity of WashingtonSeattle

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