, Volume 10, Issue 1–4, pp 247–260 | Cite as

Monoclonal antibodies identifying a novel T-Cell antigen and Ia antigens of human lymphocytes

  • John A. Hansen
  • Paul J. Martin
  • Robert C. Nowinski
Original Investigations


We describe here two new monoclonal antibodies that react with surface antigens of human lymphocytes. Antibody 7.2 identified a determinant on the framework region of the human Ia antigen. It was cytotoxic for all cultured B-cell lines, normal B cells, and monocytes. The antibody was not cytotoxic for normal T cells or for established T leukemic cell lines. In immune precipitation assays, the 7.2 antibody reacted with a bimolecular complex of two chains that resolved in polyacrylamide gels as polypeptides with molecular weights of 29000 and 34000 daltons. These precipitation results were analogous to those achieved with a rabbit antiserum prepared against human Ia antigens. Antibody 9.3 identified a determinant on the framework region of a T-cell antigen. It was cytotoxic for 50–80% of peripheral T cells and for 20–50% of thymocytes. It was not cytotoxic for cultured B-cell lines, normal B cells, or monocytes. In immune precipitation assays, the 9.3 antibody reacted with a single polypeptide with a molecular weight of 44000 daltons. Due to the expression of this antigen on a limited subpopulation of human T cells, we have designated the antigen HuLyt-1.


Molecular Weight Monoclonal Antibody Polypeptide Polyacrylamide Leukemic Cell 
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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • John A. Hansen
    • 1
    • 2
  • Paul J. Martin
    • 1
    • 2
  • Robert C. Nowinski
    • 1
    • 2
  1. 1.The Histocompatibility LaboratoryPuget Sound Blood Center, Fred Hutchinson Cancer Research CenterUSA
  2. 2.Division of Medical Oncology, Department of MedicineUniversity of WashingtonSeattle

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