Immunogenetics

, Volume 8, Issue 1, pp 499–508 | Cite as

Demonstration of Ia antigens on mouse intestinal epithelial cells by immunoferritin labeling

  • Earl L. Parr
  • Ian F. C. McKenzie
Original Investigations
Received:

Abstract

Several kinds of epithelial cells that express H-2 antigens were studied by immunoferritin labeling with an antiserum reacting only with antigens of theI region of theH-2 complex. Spleen lymphocytes were used to test the labeling system and the effect of the epithelial cell dissociation procedure on Ia antigens. Immunoglobulin-positive B10.BR lymphocytes were labeled with an anti-lak serum (A.TH anti-A.TL serum absorbed with BALB/c and B10.D2 cells), while congenic B10.D2 lymphocytes were unlabeled. The distribution of labeled Ia antigens on living B10.BR lymphocytes was patchy, while on cells fixed in periodate-lysine-paraformaldehyde before labeling, the distribution of label was continuous. Fixation evidently immobilized Ia antigens in the lymphocyte membrane. Trypsin and collagenase, as used in the epithelial cell dissociation procedure, had no discernible effect on the Ia antigens of lymphocytes. The epithelial cells studied included the columnar absorptive cells of the small intestine, uterine lining epithelium, tracheal brush cells, and pancreatic exocrine and duct cells. These cells were fixed before dissociation from their respective tissues. Ia antigens were detected only on the columnar absorptive cells of the small intestine. These cells labeled equally well with an antiserum reacting only with theK -end of theH-2 complex. In both cases, congenic control intestinal cells were unlabeled. Thus, intestinal epithelial cells appear to express theIa, K, and presumablyD regions of theH-2 complex, while the other epithelial cell types express only the K and D antigens. On fixed intestinal epithelial cells, Ia and H-2K antigens were continuously distributed on the lateral and basal cell membranes including the zonula adherens, but the antigens were absent from the apical microvillous membrane and the zonula occludens.

Keywords

Intestinal Epithelial Cell Line Epithelium Discernible Effect theI Region Epithelial Cell Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Edidin, M.: The tissue distribution and cellular location of transplantation antigens.In B. D. Kahan and R. A. Reisfeld (eds.):Transplantation Antigens; pp. 125–140, Academic Press Inc., New York, 1972Google Scholar
  2. Foker, J. E., Simmons, R. L., and Najarian, J. S.: The induction of immunity: the afferent arc.In J. S. Najarian and R. L. Simmons (eds.):Transplantation; pp. 63–94, Lea and Febiger, Philadelphia, 1972Google Scholar
  3. Hämmerling, G., Mauve, G., Goldberg, E., and McDevitt, H. O.: Tissue distribution of Ia antigens: Ia on spermatozoa, macrophages, and epidermal cells.Immunogenetics 1:428–437, 1975Google Scholar
  4. Kirby, W. N. and Parr, E. L.: The occurrence and distribution of H-2 antigens on mouse intestinal epithelial cells.J. Histochem. Cytochem. 27:746–750, 1979PubMedGoogle Scholar
  5. Lacy, P. E. and Kostianovsky, M.: Method for the isolation of intact islets of Langerhans from the rat pancreas.Diabetes 16:35–39, 1967PubMedGoogle Scholar
  6. McDevitt, H. O., Delovitch, T. L., Press. J. C., and Murphy, D. B.: Genetic and functional analysis of the Ia antigens: their possible role in retaliating the immune response.Transplant. Rev. 30:197–235, 1976PubMedGoogle Scholar
  7. McKenzie, I. F. C. and Henning, M. M.: The differential destructive and enhancing effects of anti-H-2K, H-2D, and anti-Ia antisera on murine skin allografts.J. Exp. Med. 147:611–616, 1978PubMedGoogle Scholar
  8. McLean, I. W. and Nakane, P. K.: Periodate-lysine-paraformaldehyde fixative: a new fixative for immunoelectron microscopy.J. Histochem. Cylochem. 22:1077–1084, 1974Google Scholar
  9. Parr, E. L. and Kirby, W. N.: An immunofcrritin labeling study of H-2 antigens on dissociated epithelial cells.J. Histochem. Cytochem. in press, 1979Google Scholar
  10. Parr, E. L. and Oei, J. S.: Immobilization of membrane H-2 antigens by paraformaldehyde fixation.J. Cell Biol. 59:537–542, 1973PubMedGoogle Scholar
  11. Parr, E. L.: Diversity in the expression of H-2 antigens on liver cells.Transplantation 27:45–49, 1979aPubMedGoogle Scholar
  12. Parr, E. L.: Absence of H-2 antigens from mouse pancreaticγ cells demonstrated by immunoferritin labeling.J. Exp. Med., in press, 1979 bGoogle Scholar
  13. Shreffler, D. and David, C. S.: The H-2 major histocompatibility complex and the I immune response region: genetic variation, function and organization.Adv. Immunol. 20:125–187, 1975PubMedGoogle Scholar
  14. Stingl, G., Katz, S. I., Clement, L., Green, I., and Shevach, E. M.: Immunologic functions of Ia-bearing epidermal Langerhans cells.J. Immunol. 121:2006–2011, 1978Google Scholar
  15. Takemori, T. and Tada, T.: Properties of antigen-specific suppressive T-cell factors in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.J. Exp. Med. 142:1241–50, 1975PubMedGoogle Scholar
  16. Wiman, K., Curman, B., Forsum, U., Klareskog, L., Malmnös-Tjernlund, U., Rask, L., Trägardh, L., and Peterson, P. A.: Occurrence of Ia antigens on tissues of non-lymphoid origin.Nature 276:711–13, 1978PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1979

Authors and Affiliations

  • Earl L. Parr
    • 1
  • Ian F. C. McKenzie
    • 2
  1. 1.Department of AnatomyUniversity of AdelaideAdelaideAustralia
  2. 2.The Department of MedicineAustin HospitalHeidelbergAustralia

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