Somatic Cell and Molecular Genetics

, Volume 12, Issue 1, pp 95–100 | Cite as

Human Luteinizing hormone-releasing hormone gene (LHRH) is located on short arm of chromosome 8 (region 8p11.2 → p21)

  • Teresa L. Yang-Feng
  • Peter H. Seeburg
  • Uta Francke
Brief Communication

Abstract

Luteinizing hormone-releasing hormone (LHRH) is synthesized by hypothalamic neurons and affects the release of gonadotropic hormones from the anterior pituitary gland. A cDNA clone encoding the human LHRH precursor molecule was used to assign theLHRH gene to a human chromosome by in situ hybridization and Southern blot analysis. Metaphase spreads from two normal individuals were hybridized with3H -labeled LHRH-specific sequence. Of 120 cells analyzed, 33 had silver grains over the p11.2 → p21 bands of chromosome 8. No other chromosomal site was labeled above background, indicating the presence of a single site for LHRH sequences in the human genome. Independent confirmation for this location of the humanLHRH gene on chromosome 8p was provided by analysis of DNA from human × Chinese hamster somatic cell hybrids. DNA samples were digested with EcoRI, blotted, and hybridized with the32P-labeled human LHRH precursor cDNA probe. The single 11.5-kb human-specific band was detected only in hybrids containing human chromosome 8. Also, hybridization was observed in DNA from hybrids in which a portion of human chromosome 8 (region 8pter → 8q21) had been spontaneously translocated onto a Chinese hamster chromosome.

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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Teresa L. Yang-Feng
    • 1
  • Peter H. Seeburg
    • 2
  • Uta Francke
    • 1
  1. 1.Department of Human GeneticsYale University School of MedicineNew Haven
  2. 2.Department of Molecular BiologyGenentech, Inc.South San Francisco

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