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Somatic Cell and Molecular Genetics

, Volume 12, Issue 2, pp 133–139 | Cite as

An established avian fibroblast cell line without mitochondrial DNA

  • Paul Desjardins
  • Jean -Marc de Muys
  • Réjean Morais
Article
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Abstract

tAn established avian fibroblast cell line (LSCC-H32) has been found to be inherently resistant to the growth-inhibitory effect of ethidium bromide, when supplied with exogenous urdine. After long-term exposure to ethidium bromide (90 days), the cell population has been transferred to drug-free medium for 60 days, and then seeded at low cell density. Three clones have been isolated and propagated in drug-free medium for 5, 6, and more than 12 months, respectively. It was found that none of these cell lines had detectable cytochrome c oxidase activity and that they were virtually devoid of cytochromes aa3 and b. Mitochondrial DNA was quantitated by DNA-DNA reassociation kinetics with a probe of chicken liver mitochondrial DNA. A mean number of 300 copies of mitochondrial DNA per cell was found in LSCC-H32 cells. Analysis of DNA extracted from cell populations exposed to ethidium bromide for 90 days and then transferred to drug-free medium for long periods of time revealed no mitochondrial DNA molecules by reassociation kinetics or by Southern blot hybridization of HindIII- or Aval-digested total cellular DNA.

Keywords

Cell Population Southern Blot Oxidase Activity Ethidium Bromide Blot Hybridization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Paul Desjardins
    • 1
    • 2
  • Jean -Marc de Muys
    • 1
    • 2
  • Réjean Morais
    • 1
    • 2
  1. 1.Institut du Cancer de MontréalHôpital Notre-DameMontréal
  2. 2.Département de BiochimieUniversité de MontréalMontréalCanada

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