Chromosome Research

, Volume 2, Issue 3, pp 255–256 | Cite as

Localization of uridine monophosphate synthase (UMPS) gene to river buffalo chromosomes by FISH

  • L. Iannuzzi
  • G. P. Di Meo
  • A. M. Ryan
  • D. S. Gallagher
  • L. Ferrara
  • J. E. Womack
Short Communication

Abstract

Uridine monophosphate synthase plays an important role in pyrimidine synthesis, converting orotic acid to uridine 5′ monophosphate. In cattle,UMPS deficiency is inherited as a monogenic autosomal recessive trait. While heterozygous carriers are phenotypically normal, homozygotes are lethalin utero (Shankset al. 1992).UMPS has been mapped to human chromosome 3q13 (Qumsiyehet al. 1989), sheep chromosome 1q (Burkinet al. 1993) and cattle chromosome 1q31 (Ryanet al. 1994). In the present study we used a cattle genomic probe to localizeUMPS to river buffalo chromosomes by fluorescencein situ hybridization.

Key words

Bubalus bubalis chromosomes FISH river buffalo UMPS 

References

  1. Burkin DJ, Morse HG, Broad TE, Pearce PD, Ansari HA, Lewis PE, Jones C (1993) Mapping the sheep genome: production of characterized sheep × hamster cell hybrids.Genomics 16: 466–472.PubMedGoogle Scholar
  2. Groenen MAM, van der Poel JJ, Dijkhof EJM, Giphart MF (1989) Cloning of the bovine major histocompatibility complex class II genes.Anim Genet 20: 267–278.PubMedGoogle Scholar
  3. Iannuzzi L, Di Meo GP, Perucatti A, Ferrara L (1990) The high resolution G-and R-banding patterns in chromosomes of river buffalo (Bubalus bubalis L.).Hereditas 112: 209–215.PubMedGoogle Scholar
  4. Iannuzzi L, Gallagher DS, Ryan AM, Di Meo GP, Womack JE (1993) Chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential R-banding and fluorescentin situ hybridization.Cytogenet Cell Genet 62: 225–227.Google Scholar
  5. Qumsiyeh MB, Valentine MB, Suttle DP (1989) Localization of the gene for uridine monophosphate synthase to human chromosome region 3q13 byin situ hybridization.J Hered 81: 111–116.Google Scholar
  6. Ryan AM, Gallagher DS, Schober S, Schwenger B, Womack JE (1994) Somatic cell mapping andin situ localization of the bovine uridine monophosphate synthase gene (UMPS).Mamm Genom,5: 46–47.Google Scholar
  7. Shanks RD, Popp RG, McCoy GC, Nelson DR, Robinson JL (1992) Identification of the homozygous recessive genotype for the deficiency of uridine monophosphate syntase in 35-day bovine embryos.J Reprod Fert 94: 5–10.Google Scholar
  8. Womack JE, Bolch SN, Fries R (1993) Gene map of the cow (Bos Taurus) 2n = 60. In: O'Brien S. J. ed.Genetic Maps, Vol7, Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press. pp. 4.264–4.275.Google Scholar

Copyright information

© Rapid Communications of Oxford Ltd 1994

Authors and Affiliations

  • L. Iannuzzi
    • 1
  • G. P. Di Meo
    • 1
  • A. M. Ryan
    • 2
  • D. S. Gallagher
    • 2
  • L. Ferrara
    • 1
  • J. E. Womack
    • 2
  1. 1.National Research Council (CNR)I.A.B.B.A.M.Naples-PonticelliItaly
  2. 2.Department of Veterinary Pathobiology and Center for Animal Genetics, Institute of Biosciences and TechnologyTexas A & M UniversityCollege StationUSA

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