Somatic Cell Genetics

, Volume 3, Issue 2, pp 231–236 | Cite as

A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

  • Malcolm L. Gefter
  • David H. Margulies
  • Matthew D. Scharff
Brief Communication


A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentration of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.


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Copyright information

© Plenum Publishing Corp 1977

Authors and Affiliations

  • Malcolm L. Gefter
    • 1
  • David H. Margulies
    • 1
  • Matthew D. Scharff
    • 1
  1. 1.Department of Cell BiologyAlbert Einstein College of MedicineBronx
  2. 2.Department of BiologyMassachusetts Institute of TechnologyCambridge

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