Somatic Cell Genetics

, Volume 3, Issue 1, pp 47–60 | Cite as

Somatic cell genetic assignment of the human gene for mitochondrial NADP-linked isocitrate dehydrogenase to the long arm of chromosome 15

  • Nobuyoshi Shimizu
  • Richard E. Giles
  • Raju S. Kucherlapati
  • Yoshiko Shimizu
  • Frank H. Ruddle
Article

Abstract

A double-immunodiffusion method has been developed to detect human mitochondrial NADP-linked isocitrate dehydrogenase (EC 1.1.1.42; designated as IDH-2) using rabbit antiserum against the relevant enzyme. The method allows one to distinguish human IDH-2 from its mouse counterpart in extracts from human-mouse somatic cell hybrids. A correlation was found between the expression of human IDH-2 and the presence of human chromosome 15 in a “panel” of eight independent hybrid clones. Analysis of human marker enzymes for 37 different clones revealed a syntenic relationship between IDH-2 and mannose phosphate isomerase (EC 5.3.1.8; MPI), which has been assigned to chromosome 15 (1). These results permit the assignment of the structural gene for human IDH-2 to human chromosome 15. IDH-2 and human cytoplasmic IDH (IDH-1) were found to be asyntenic. Evidence from hybrid clones carrying a human X/15 translocation chromosome indicates that the human IDH-2 gene can be localized to the q11-qter region of chromosome 15.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    van Heyningen, V., Bobrow, M., Bodmer, W.F., Gardiner, S.E., Povey, S., Hopkinson, D.A. (1975).Ann. Hum. Genet. 38:295–303.PubMedGoogle Scholar
  2. 2.
    Plaut, G.W.E. (1963). InThe Enzymes (Boyer, P.D., Lardy, H.A., and Myrback, K., eds.), 2nd Ed., Vol. 7, pp. 105–126, Academic Press, New York.Google Scholar
  3. 3.
    Turner, B.M., Fisher, R.A., and Harris, H. (1974).Ann. Hum. Genet. London 39:455–467.Google Scholar
  4. 4.
    Ruddle, F.H., and Creagan, R.P. (1975).Ann. Rev. Genet. 9:407–486.PubMedGoogle Scholar
  5. 5.
    Creagan, R.P., Carritt, B., Chen, S., Kucherlapati, R.S., McMorris, F.A., Ricciuti, F., Tan, Y.H. Tischfield, J. A., and Ruddle, F.H. (1974),Am. J. Hum. Genet. 26:604–613.PubMedGoogle Scholar
  6. 6.
    Schatz, G., and Mason, T.L. (1974).Ann. Rev. Biochem. 43:51–87.Google Scholar
  7. 7.
    Henderson, N.S. (1965).J. Exp. Zool. 185:263–274.Google Scholar
  8. 8.
    Chapman, V.M., Ruddle, F. H., and Roderick, T. H. (1970).Biochem. Genet. 5:101–110.Google Scholar
  9. 9.
    Hutton, J.J., and Roderick, T.H. (1970).Biochem. Genet. 4:339–350.PubMedGoogle Scholar
  10. 10.
    Chen, S-H., Fossum, B.L.G., and Giblett, E.R. (1972).Am. J. Hum. Genet. 24:325–329.PubMedGoogle Scholar
  11. 11.
    Ashwell, M., and Work, T.S. (1970).Ann. Rev. Biochem. 39:251–290.PubMedGoogle Scholar
  12. 12.
    Grzeschik, K-H. (1976). InHuman Gene Mapping 3, Baltimore Conference, Bergsma, D. (ed.), The National Foundation-March of Dimes, pp. 142–148.Google Scholar
  13. 13.
    Tischfield, J.A., and Ruddle, F.H. (1974).Proc. Natl. Acad. Sci. USA 71:45–49.PubMedGoogle Scholar
  14. 14.
    Ruddle, F.H., Chapman, V.M., Chen, T.R., and Klebe, R.J. (1970).Nature 227:251–257.PubMedGoogle Scholar
  15. 15.
    Satlin, A., Kucherlapati, R.S., and Ruddle, F.H. (1975).Cytogenet. Cell Genet. 15:146–152.PubMedGoogle Scholar
  16. 16.
    Shimizu, N., Kucherlapati, R.S., and Ruddle, F.H. (1976).Somat. Cell Genet.,2:345–357.PubMedGoogle Scholar
  17. 17.
    Puck, T.T., and Marcus, P.I. (1955).Proc. Natl. Acad. Sci. USA 41:432–437.Google Scholar
  18. 18.
    Eagle, H. (1955).Proc. Soc. Exp. Biol. Med. 89:362.PubMedGoogle Scholar
  19. 19.
    Littlefield, J.W. (1966).Exp. Cell Res. 41:190–196.PubMedGoogle Scholar
  20. 20.
    Puck, T.T., Cieciura, S.J., and Robinson, A. (1958).J. Exp. Med. 108:945–955.PubMedGoogle Scholar
  21. 21.
    Shimizu, N., Shimizu, Y., Kucherlapati, R.S., and Ruddle, F.H. (1976).Cell 7 123–130.PubMedGoogle Scholar
  22. 22.
    Ouchterlony, O. (1949).Acta Pathol. Microbiol. Scand. 26:507.Google Scholar
  23. 23.
    Nichols, E.A., and Ruddle, F.H. (1973).J. Histochem. Cytochem. 21:1066–1081.PubMedGoogle Scholar
  24. 24.
    Hsu, T.C., and Pomerat, C.M. (1953).Heredity 44:23–29.Google Scholar
  25. 25.
    Caspersson, T., Zech, L., Johansson, C., and Modest, E.J. (1970).Chromosoma 30:215–227.PubMedGoogle Scholar
  26. 26.
    Westerveld, A., Van Someren, H., Beyersbergen van Henegouwen, H.M.A., and Oosterbaan, K.A. (1975). InHuman Gene Mapping 2, Rotterdam Conference 1974, Bergsma, D. (ed.). The National Foundation-March of Dimes, pp. 285–287.Google Scholar
  27. 27.
    Littlefield, J.W. (1964).Science 145:709–710.PubMedGoogle Scholar
  28. 28.
    Ricciuti, F., and Ruddle, F.H. (1973).Nature New Biol. 241:180–182.PubMedGoogle Scholar
  29. 29.
    Meera Khan, P., Los, W.R.T., Pearson, P.L., Westerveld, A, and Bootsma, D. (1974).Hum. Hered. 24:415–423.PubMedGoogle Scholar
  30. 30.
    Craig, I., Tolley, E., and Bobrow, M. (1976). InHuman Gene Mapping 3, Baltimore Conference, Bergsma, D. (ed.), The National Foundation-March of Dimes, pp. 118–121.Google Scholar
  31. 31.
    Westerveld, A., Beyersbergen van Henegouwen, H.M.A., and van Someren, H. (1975). InHuman Gene Mapping 2, Rotterdam Conference, Bergsma, D. (ed.), The National Foundation March of Dimes, pp. 283–284.Google Scholar
  32. 32.
    van Heyningen, V., Bobrow, M., Bodmer, W.F., Gardiner, S.E., Povey, S., and Hopkinson, D.A. (1975).Ann. Hum. Genet. Lond. 38:295–303.Google Scholar
  33. 33.
    Willecke, K., Rever, T., Kucherlapati, R.S., and Ruddle, F.H. (1976). InHuman Gene Mapping 3, Baltimore Conference, Bergsma, D. (ed.), The National Foundation-March of Dimes, pp. 252–255.Google Scholar
  34. 34.
    van Heyningen, V., Craig, I, and Bodmer, W.F. (1973).Nature 242:509–513.PubMedGoogle Scholar
  35. 35.
    van Someren, H., Beyersbergen van Henegouwen, H.M.A., Westerveld, A., and Bootsma, D. (1974).Cytogenet. Cell Genet. 13:551–557.PubMedGoogle Scholar
  36. 36.
    Povey, S., Slaughter, C.A., Wilson, D.E., Jr., Buchton, K., Gormley, I.P., and Perry, P. (1976). InHuman Gene Mapping 3, Baltimore Conference, Bergsma, D. (ed.). The National Foundation-March of Dimes, pp. 211–214.Google Scholar
  37. 37.
    Creagan, R.P., Carritt, B., Chen, S., Kucherlapati, R.S., McMorris, F.A., Ricciuti, F., Tan, Y.H., Tischfield, J.A., and Ruddle, F.H. (1974).Am. J. Hum. Genet. 26:604–613.PubMedGoogle Scholar
  38. 38.
    Miller, O.J., Allderdice, P.W., Miller, D.A., Berg, W.R., and Migeon, B.R. (1971).Science 173:244–245.PubMedGoogle Scholar
  39. 39.
    Kislev, N., Spolsky, C.M., and Eisenstadt, J.M. (1973).J. Cell Biol. 57:571–579.PubMedGoogle Scholar
  40. 40.
    Attardi, B., and Attardi, G. (1972).Proc. Natl. Acad. Sci. USA 69:129–133.PubMedGoogle Scholar
  41. 41.
    Clayton, D.A., Teplitz, R.L., Nabholz, M., Dovey, H., and Bodmer, W. (1971).Nature 234:560–562.PubMedGoogle Scholar
  42. 42.
    Coon, H.G., Horak, J., and David, J.B. (1973).J. Mol. Biol. 81:285–298.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Nobuyoshi Shimizu
    • 1
  • Richard E. Giles
    • 1
  • Raju S. Kucherlapati
    • 2
  • Yoshiko Shimizu
    • 1
  • Frank H. Ruddle
    • 1
  1. 1.Department of BiologyYale UniversityNew Haven
  2. 2.Department of Biochemical Sciences, Moffett LaboratoryPrinceton UniversityPrinceton

Personalised recommendations