Somatic Cell Genetics

, Volume 6, Issue 3, pp 407–418 | Cite as

Large-scale isolation of UV-sensitive clones of CHO cells

  • David B. Busch
  • James E. Cleaver
  • Donald A. Glaser
Article
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Revised:

Abstract

We have isolated 54 ultraviolet light (UV) sensitive clones of Chinese hamster ovary (CHO) cells, including two from a parent cell line which is hypersensitive to ethyl methanesulfonate (EMS) and is also sensitive to X rays. A replica plating technique was used for the isolation of two of the clones, and a semiautomated technique was used for the isolation of the other 52 clones. We have observed UV sensitization of up to 5-fold in the mutants relative to the parent in terms of the slopes of the survival curves. Seven of the clones were examined for DNA repair competence using a repair replication assay, and all exhibited a DNA repair defect resembling that seen in human mutant xeroderma pigmentosumcells. We have also demonstrated an approximately 9-fold enhancement in the UV mutagenic response of two of the repair replication-defective clones relative to the parent for resistance to ouabain, 6-thioguanine, and 8-azaadenine.

Keywords

Ouabain Parent Cell Chinese Hamster Ovary Methanesulfonate Parent Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Literature cited

  1. 1.
    Cleaver, J. E. (1968).Nature 218:652–656.Google Scholar
  2. 2.
    Cleaver, J. E. (1978). InMetabolic Basis of Inherited Disease, 4th ed.(eds.) Stanbury, J. B., Weingarden, J. B., and Frederickson, D. S. (McGraw Hill, New York), pp. 1072–1095.Google Scholar
  3. 3.
    Paterson, M. C., Smith, B. P., Lohman, P. H. M., Anderson, A. K., and Fishman, L. (1976).Nature 260:444–447.Google Scholar
  4. 4.
    Fujiwara, Y., Tatsumi, M., and Sasaki, M. S. (1977).J. Mol. Biol. 113:635–649.Google Scholar
  5. 5.
    Cleaver, J. E. (1978).Biochim. Biophys. Acta 516:489–516.Google Scholar
  6. 6.
    Sinclair, W. K. (1964).Radiat. Res. 21:584–611.Google Scholar
  7. 7.
    Todd, P. W., and Hellewell, A. B. (1969).Mutat. Res. 7:129–132.Google Scholar
  8. 8.
    Isomura, K., Nikaido, O., Horikawa, M., and Sugahara, T. (1973).Radiat. Res. 53:143–152.Google Scholar
  9. 9.
    Stamato, T. D., and Waldren, C. A. (1977).Somat. Cell Genet. 3:431–440.Google Scholar
  10. 10.
    Sato, K., and Hieda, N. (1979).Int. J. Radiat. Biol. 35:83–87.Google Scholar
  11. 11.
    Kuroki, T., and Miyashita, S. Y. (1977).J. Cell Physiol. 90:79–90.Google Scholar
  12. 12.
    Shiomi, T., and Sato, K. (1979).Somat. Cell Genet. 5:193–201.Google Scholar
  13. 13.
    Rosenstein, B., and Ohlsson-Wilhelm, B. M. (1979).Somat. Cell Genet. 5:117–128.Google Scholar
  14. 14.
    Schultz, R., Chang, C-C., and Trosko, J. E.Proc. AACR ASCO (1978).Google Scholar
  15. 15.
    Thompson, L. H., Rubin, J. S., Cleaver, J. E., Whitmore, G. F., and Brookman, K. W.Somat. Cell Genet. 6:391–405.Google Scholar
  16. 16.
    Thompson, L. H., Fong, S., and Brookman, K. (1980).Mutat. Res. 74:21–36.Google Scholar
  17. 17.
    Konrad, M. W., Storrie, B., Glaser, D. A., and Thompson, L. H. (1977).Cell 10:305–312.Google Scholar
  18. 18.
    Dulbecco, R., and Vogt, M. (1954).J. Exp. Med. 99:167–182.Google Scholar
  19. 19.
    O'Neill, J. P., and Hsie, A. W. (1977).Nature 269:815–816.Google Scholar
  20. 20.
    O'Neill, J. P., Fuscoe, J. C., and Hsie, A. W. (1978).Cancer Res. 38:506–509.Google Scholar
  21. 21.
    Cleaver, J. E. (1973).Cancer Res. 33:362–369.Google Scholar
  22. 22.
    Cleaver, J. E. (1977). InHandbook of Mutagenicity Test Procedures, (eds.) Kilbey, B., Legator, M., Nichols, W., and Ramel, C. (Elsevier, North Holland), pp. 19–28Google Scholar
  23. 23.
    Russell, W. C., Newman, C., and Williamson, D. H. (1975).Nature 253:461–462.Google Scholar
  24. 24.
    Baker, R. M., Brunette, D. M., Mankovitz, R., Thompson, L. H., Whitmore, G. F., Siminovitch, L., and Till, J. E. (1974).Cell 1:9–21.Google Scholar
  25. 25.
    Esko, J. D., and Raetz, C. R. H. (1978).Proc. Natl. Acad. Sci. 75:1190–1193.Google Scholar
  26. 26.
    Sevastopoulos, C. G., Wehr, C. T., and Glaser, D. A. (1977).Proc. Natl. Acad. Sci. 74:3485–3489.Google Scholar
  27. 27.
    Gautschi, J. R., Young, B. R., and Cleaver, J. E. (1973).Exp. Cell Res. 76:87–94.Google Scholar
  28. 28.
    Maher, V. M., and McCormick, J. J. (1976). InBiology of Radiation Carcinogenesis, (eds.) Yuhas, J. M., Tennant, R. W., and Regan, J. D. (Raven Press, New York), pp. 129–45.Google Scholar
  29. 29.
    Maher, V. M., Ouellette, L. M., Curren, R. D., and McCormick, J. J. (1976).Nature 261:593–595.Google Scholar
  30. 30.
    Howard-Flanders, P., and Boyce, R. P. (1966).Rad. Res. Suppl. 6:156–184.Google Scholar
  31. 31.
    Game, J. C., and Cox, B. S. (1972).Mutat. Res. 16:353–362.Google Scholar
  32. 32.
    Game, J. C., and Cox, B. S. (1973).Mutat. Res. 20:35–44.Google Scholar
  33. 33.
    Brookman, K. W., Busch, D. B., Thompson, L. H., Mooney, C. L., and Glaser, D. A. (1980). Eleventh Annual Meeting Environmental Mutagen Society, abstract, p. 127.Google Scholar
  34. 34.
    Illmensee, K., and Mintz, B. (1976).Proc. Natl. Acad. Sci. 73:549–553.Google Scholar
  35. 35.
    Ames, B. N. (1979).Science 204:587–593.Google Scholar
  36. 36.
    O'Neill, J. P., Couch, D. B., Machanoff, R., San Sebastian, J. R., Brimer, P. A., and Hsie, A. W. (1977).Mutat. Res. 45:103–109.Google Scholar

Copyright information

© Plenum Publishing Corporation 1980

Authors and Affiliations

  • David B. Busch
    • 1
  • James E. Cleaver
    • 2
  • Donald A. Glaser
    • 1
  1. 1.Department of Molecular BiologyUniversity of CaliforniaBerkeley
  2. 2.Laboratory of RadiobiologyUniversity of CaliforniaSan Francisco

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