Journal of Clinical Immunology

, Volume 16, Issue 4, pp 222–229

Tubing loops as a model for cardiopulmonary bypass circuits: Both the biomaterial and the blood-gas phase interfaces induce complement activation in anin vitro model

  • Jian Gong
  • Rolf Larsson
  • Kristina Nilsson Ekdahl
  • Tom Eirik Mollnes
  • Ulf Nilsson
  • Bo Nilsson
Original Articles

DOI: 10.1007/BF01541228

Cite this article as:
Gong, J., Larsson, R., Ekdahl, K.N. et al. J Clin Immunol (1996) 16: 222. doi:10.1007/BF01541228

Abstract

We describe here a model for the study of blood/surface and blood/air interaction as encountered in cardiopulmonary bypass (CPB) circuits. Polyethylene tubing was filled with serum or blood and closed end to end into loops whereby the volume of the remaining air bubble was inversely varied with respect to that of the fluid. The loops were rotated vertically in a water bath at 37°C. The profiles of C3a, iC3, and TCC generation were similar to those observed at surgery, involving CPB. Soluble heparin and heparan sulfate inhibited both C3a and TCC formation, but surface-conjugated heparin had only a minor effect. Binding of C3 and/or C3 fragments to the heparin surface was much reduced compared to the amine matrix to which heparin was linked, but compared with the polyethylene surface the effect was less pronounced. These data suggest that, in addition to the biomaterial surface, the blood-gas interface seems to play an important role in the activation of complement and that this activation is inhibitable by high concentrations of soluble glucose aminoglycans.

Key words

Complement activation C3a terminal sC5b-9 cardiopulmonary bypass heparin 

Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Jian Gong
    • 1
  • Rolf Larsson
    • 1
  • Kristina Nilsson Ekdahl
    • 1
  • Tom Eirik Mollnes
    • 2
    • 3
  • Ulf Nilsson
    • 1
  • Bo Nilsson
    • 1
  1. 1.Department of Clinical Immunology and Transfusion MedicineUniversity HospitalUppsalaSweden
  2. 2.Department of Immunology and Transfusion MedicineNordland Central HospitalBodø
  3. 3.University of TromsøTromsøNorway

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