Clinical and biological significance of interleukin-10 plasma levels in patients with septic shock
- 70 Downloads
Interleukin-10 is a potent macrophage-deactivating cytokine that inhibits lipopolysaccharide-induced tumor necrosis factor production. We determined the plasma levels of immunoreactive interleukin-10 in 16 patients with septic shock and in 11 patients with circulatory shock of nonseptic origin. In septic shock, interleukin-10 levels peaked during the first 24 h (median: 48 pg/ml) and decreased progressively till Day 5. In nonseptic shock, interleukin-10 plasma levels also increased during the first 24 h but to a lesser extent (median: 17 pg/ml). In septic shock patients, interleukin-10 plasma levels were positively correlated with tumor necrosis factor (r=0.8,p=0.01) and with parameters of shock severity including lactate levels (r=0.56, p<0.05) and correlated negatively with blood platelet counts (r=−0.65,p<0.05). The decreased production of tumor necrosis factor-α and interleukin-6 afterin vitro incubation of whole blood from septic shock patients with lipopolysaccharide was not influenced byin vitro neutralization of interleukin-10. We conclude that interleukin-10 is produced in patients with circulatory shock of septic and nonseptic origin and that the production of this anti-inflammatory cytokine during septic shock correlates positively with the intensity of the inflammatory response.
Key wordsSeptic shock interleukin-10 tumor necrosis factor lipopolysaccharide monocyte
Unable to display preview. Download preview PDF.
- 13.De Groote D, Marchant A, Fauchet F, Jadoul M, Dehart I, Gérard C, Gevaert Y, Lopez M, Gathy R, Franssen JD, Radoux D, Franchimont P: Characterisation of monoclonal antibodies against human interleukin-10 and their use in an ELISA for the measurement of this cytokine. J Immunol Meth 177:225–234, 1994Google Scholar
- 18.van der Poll T, Marchant A, Berman L, Lazarus DD, Keogh CV, Nguyen L, Goldman M, Moldawer LL, Lowry SF: Endogenous interleukin-10 protects against death in septic peritonitis in mice. Surg Forum (in press)Google Scholar
- 24.Moore KW, O'Garra A, de Waal Malefyt R, Vieira P, Mosmann TR: Interleukin-10. Ann Rev Immunol 11:165–190, 1993Google Scholar
- 34.Basha MA, Meyer GS, Kunkel SL, Strieter RM, Rivers EP, J Popovich: Presence of tumor necrosis factor in humans undergoing cardiopulmonary resuscitation with return of spontaneous circulation. J Crit Care 6:185–189, 1991Google Scholar