Journal of tissue culture methods

, Volume 16, Issue 3–4, pp 189–192 | Cite as

Cytoscription: Computer controlled micropositioning of cell adhesion proteins and cells

  • Robert J. Klebe
  • Charles A. Thomas
  • George M. Grant
  • Anne Grant
  • Paramita Gosh
Preparation Of Adhesive Surfaces


Methods are described for the computer controlled micropositioning of both mammalian cells and cell adhesion proteins. Cell adhesion proteins can be micropositioned by using cell adhesion proteins instead of ink in an inkjet printer. Cells can be positioned with a computer controlled X-Y translation table and a fluorescence activated cell sorter (FACS).

Key words

Cell adhesion Cell adhesion proteins Computers Fibronectins Morphogenesis Tissue engineering 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Anonymous (1990). Parker Daedal Catalogue. Daedal 25M-11/90: 1∓96.Google Scholar
  2. 2.
    Akiyama SK, Nagata K, Yamada KM (1990). Cell surface receptors for extracellular matrix components. Biochim Biophys Acta 1031: 91–110.PubMedGoogle Scholar
  3. 3.
    Bentley K, Klebe RJ (1985). Fibronectin binding properties of bacteriological petri plates and tissue culture dishes. J Biomed Mater Res 19: 757–769.PubMedGoogle Scholar
  4. 4.
    Edelman GM, Jones FS (1993). Outside and downstream of the homeobox. J Biol Chem 268: 20683–20686.PubMedGoogle Scholar
  5. 5.
    Gehring WJ (1993). Exploring the homeobox. Gene 135: 215–221.PubMedGoogle Scholar
  6. 6.
    Klebe RJ (1974). Isolation of a collagen-dependent cell attachment factor. Nature 250: 248–251.PubMedGoogle Scholar
  7. 7.
    Klebe RJ (1988). Cytoscribing: A method for micropositioning cells and the construction of three-dimensional tissues. Exp Cell Res 179: 362–373.PubMedGoogle Scholar
  8. 8.
    Klebe RJ, Bentley KL, Schoen RC (1981). Adhesive substrates for fibronectin. J Cell Physiol 109: 481–488.PubMedGoogle Scholar
  9. 9.
    Miekka S, Ingham K, Menache D (1989). Rapid methods for isolation of human plasma fibronectin. Thromb Res 27: 1–14.Google Scholar
  10. 10.
    Ruoslahti E (1991). Integrins. J Clin Invest 87: 1–5.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Robert J. Klebe
    • 2
  • Charles A. Thomas
    • 1
  • George M. Grant
    • 2
  • Anne Grant
    • 2
  • Paramita Gosh
    • 2
  1. 1.Department of MicrobiologyUniversity of Texas Health Science CenterSan AntonioUSA
  2. 2.Department of Cellular and Structural BiologyThe University of Texas Health Science CenterSan AntonioUSA

Personalised recommendations