Somatic Cell and Molecular Genetics

, Volume 12, Issue 5, pp 449–458 | Cite as

Mitochondrial protein synthesis in interspecific somatic cell hybrids

  • S. H. Zuckerman
  • F. P. Gillespie
  • J. F. Solus
  • R. Rybczynski
  • J. M. Eisenstadt
Article
Received:
Revised:

Abstract

The fusion of an oligomycin (OLI) -resistant mutant of mouse LM(TK−) cells to a chloramphenicol (CAP) -resistant mutant of AK412 Chinese hamster cells resulted in a series of interspecific somatic cell hybrids. Hybrids selected in HAT medium retained only mouse mitochondrial genomes while hybrids selected in HA T plus CAP and OLI retained both hamster and mouse mitochondrial genomes in approximately equal amounts. Nuclear-coded mitochondrial proteins from both parental species were incorporated into mitochondria in all of the hybrids. However, the mitochondrially coded proteins of three individually isolated hybrid cell lines were predominantly mouse-specific, with only trace amounts of hamster protein detected.

Keywords

Protein Synthesis Somatic Cell Chloramphenicol Trace Amount Mitochondrial Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • S. H. Zuckerman
    • 1
  • F. P. Gillespie
    • 2
  • J. F. Solus
    • 3
  • R. Rybczynski
    • 4
  • J. M. Eisenstadt
    • 2
  1. 1.Department of ImmunologyLilly Research LaboratoriesIndianapolis
  2. 2.Department of Human GeneticsYale University School of MedicineNew Haven
  3. 3.Department of BiologyUniversity of CaliforniaSan Diego, La Jolla
  4. 4.Section of Molecular NeurobiologyYale University School of MedicineNew Haven

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