Somatic Cell Genetics

, Volume 3, Issue 3, pp 303–312 | Cite as

Fusion of T and B cells

  • Georges Köhler
  • Terry Pearson
  • César Milstein


Hybrid cells were prepared by fusing an immunoglobulinsecreting mouse myeloma line (B cell) with an allogeneic T-cell lymphoma which expresses the surface antigen Thy 1. The resulting hybrids expressed H2 antigens of both parental cells and secreted the immunoglobulin of the myeloma parent but did not express the Thy 1 antigen of the lymphoma parent. Twenty-one hybrids were formed from fusion of the same myeloma line with TNP-SRBC-primed spleen cells. Most of the hybrid lines exhibited characteristics expected for the fusion of the myeloma to B lymphocytes. No hybrids between the myeloma line and spleen T cells were identified as none of the hybrids expressed the T-cell-specific antigen Thy 1. We discuss possible reasons for failure to produce hybrids with T-cell characteristics in these types of fusion.


Lymphoma Myeloma Surface Antigen Spleen Cell Parental Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    Kiesielow, P., Hirst, J. A., Shiku, H., Beverly, P. C. L., Hoffman, M. K., Boyse, E. A., and Oettgen, H. F. (1975).Nature 253:219–220.PubMedGoogle Scholar
  2. 2.
    Beverly, P. C. L. (1977). InB and T Cells in Immune Recognition, (eds.) Loor, F., and Roelants, G. E., London, Wiley & Sons, in press.Google Scholar
  3. 3.
    Loor, F., and Roelants, G. E. (1975).Ann. N. Y. Acad. Sci. 245:226–241.Google Scholar
  4. 4.
    Raff, M. C. (1971).Transplant. Rev. 6:52–80.PubMedGoogle Scholar
  5. 5.
    Potter, M. (1972).Physiol. Rev. 52:631–719.PubMedGoogle Scholar
  6. 6.
    Köhler, G., and Milstein, C. (1976).Eur. J. Immunol. 6:511–519.PubMedGoogle Scholar
  7. 7.
    Köhler, G., and Milstein, C. (1975).Nature 256:495–497.PubMedGoogle Scholar
  8. 8.
    Packman, R., and Merler, E. (1973).Nature (London), New Biol. 245:14–16.Google Scholar
  9. 9.
    Hyman, R., and Kelleher, T. (1975).Somat. Cell Genet. 1:335–343.PubMedGoogle Scholar
  10. 10.
    Liang, W., and Cohen, E. P. (1975).Proc. Natl. Acad. Sci. USA 72:1873–1877.PubMedGoogle Scholar
  11. 11.
    Hyman, R. (1973).J. Natl. Cancer Inst. 50:415–422.PubMedGoogle Scholar
  12. 12.
    Hyman, R., and Stallings, V. (1974).J. Natl. Cancer Inst. 52:429–436.PubMedGoogle Scholar
  13. 13.
    Littlefield, J. W. (1964).Science 145:709–710.PubMedGoogle Scholar
  14. 14.
    Cotton, R. G. H., Secher, D. S., and Milstein, C. (1973).Eur. J. Immunol. 3:136–140.Google Scholar
  15. 15.
    Jerne, N. K., and Nordin, A. A. (1963).Science 140:403–404.PubMedGoogle Scholar
  16. 16.
    Awdeh, A. L., Williamson, A. R., and Asconas, B. A. (1968).Nature 219:66–67.PubMedGoogle Scholar
  17. 17.
    Köhler, G. Howe, S. C., and Milstein, C. (1976).Eur. J. Immunol. 6:292–295.PubMedGoogle Scholar
  18. 18.
    Nowinski, R. C., and Klein, P. A. (1975).J. Immunol. 115:1261–1268.PubMedGoogle Scholar
  19. 19.
    Demant, P. (1973).Transplant. Rev. 15:164–165.Google Scholar
  20. 20.
    Sachs, D. H., David, C. S., Shreffler, D. C., Nathanson, S. G., and McDevitt, H. O. (1975).Immunogenetics 2:301–312.Google Scholar
  21. 21.
    Lefkovits, I. (1974).Curr. Top. Microbiol. Immunol. 65:21.PubMedGoogle Scholar
  22. 22.
    Plotz, P. H., Talal, N., and Asofky, R. (1968).J. Immunol. 100:744–751.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Georges Köhler
    • 1
  • Terry Pearson
    • 1
  • César Milstein
    • 1
  1. 1.MRC Laboratory of Molecular BiologyCambridgeEngland

Personalised recommendations