Somatic Cell Genetics

, Volume 3, Issue 3, pp 237–245 | Cite as

Human mitochondrial thymidine kinase is coded for by a gene on chromosome 16 of the nucleus

  • K. Willecke
  • T. Reber
  • R. S. Kucherlapati
  • F. H. Ruddle


The expression of human mitochondrial thymidine kinase (mt TK) was investigated by polyacrylamide electrophoresis in 19 independent human—mouse somatic cell hybrids which allowed all human chromosomes to be analyzed. In 8 hybrid clones the presence of this enzymatic activity could be demonstrated. Human mt TK segregated concordantly with human adenine phosphoribosyltransferase (APRT) and human chromosome 16. Discordant segregation with all other human chromosomes was demonstrated by karyotype and isozyme analyses. These results suggest that human mt TK is coded for by a gene on chromosome 16 of the nucleus. Thus human mt TK is genetically different from human cytosol thymidine kinase which is coded for by a gene on chromosome 17. The appearance of one heteropolymer band after electrophoretic separation of human and murine mt TK supports the notion that both enzymes have dimeric structures.


Enzymatic Activity Adenine Somatic Cell Thymidine Human Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    Kit, S., Leung, W.-C, and Kaplan, L. A. (1973).Eur. J. Biochem. 39:43–48.PubMedGoogle Scholar
  2. 2.
    Kit, S., Leung, W.-C, and Trkula, D. (1974). InControl Processes in Neoplasia, (eds.) Mehlman, M. A., and Hanson, R. W., pp. 103–145, Academic Press, New York.Google Scholar
  3. 3.
    Adler, R., and McAuslan, R. (1974).Cell 2:113–117.PubMedGoogle Scholar
  4. 4.
    Elsevier, S. M., Kucherlapati, R. S., Nichols, E. A., Creagan, R. P., Giles, R. E., Ruddle, F. H., Willecke, K., and McDougall, J. K. (1974).Nature 251:633–635.PubMedGoogle Scholar
  5. 5.
    Kit, S., and Leung, W.-C. (1975).Biochem. Biophys. Res. Commun. 67:1–7.PubMedGoogle Scholar
  6. 6.
    Kit, S., and Leung, W.-C. (1974).J. Cell Biol. 61:35–44.Google Scholar
  7. 7.
    Attardi, B., and Attardi, G. (1972).Proc. Natl. Acad. Sci. USA 69:129–133.PubMedGoogle Scholar
  8. 8.
    Coon, H. G., Horack, I., and David, I. B. (1973).J. Mol. Biol. 81:285–298.PubMedGoogle Scholar
  9. 9.
    Willecke, K., Reber, T., Kucherlapati, R., and Ruddle, F. H. (1976).Cytogenet. Cell Genet. 16:252–255.PubMedGoogle Scholar
  10. 10.
    Kusano, T., Long, C., and Green, H. (1971).Proc. Natl. Acad. Sci. USA 68:82–86.PubMedGoogle Scholar
  11. 11.
    Tischfield, J. A., and Ruddle, F. H. (1974).Proc. Natl. Acad. Sci. USA 71:45–49.PubMedGoogle Scholar
  12. 12.
    Giles, R. E., and Ruddle, F. H. (1973). InTissue Culture—Methods and Applications, (eds.) Kruse, P. F., and Patterson, M. K., pp. 475–500, Academic Press, New York.Google Scholar
  13. 13.
    Littlefield, J. W. (1966).Exp. Cell Res. 41:190–196.PubMedGoogle Scholar
  14. 14.
    Kit, S., Dubbs, D. R., Piekarski, L. J., and Hsu, T. C. (1963).Exp. Cell Res. 31:297–312.PubMedGoogle Scholar
  15. 15.
    Chen, S., Creagan, R. P., Nichols, E. A., and Ruddle, F. H. (1975).Cytogenet. Cell Genet. 14:269–272.PubMedGoogle Scholar
  16. 16.
    Willecke, K., Lange, R., Krüger, A., and Reber, T. (1976).Proc. Natl. Acad. Sci. USA 73:1274–1278.PubMedGoogle Scholar
  17. 17.
    Nichols, E. A., and Ruddle, F. H. (1973).J. Histochem. Cytochem. 21:1066–1081.PubMedGoogle Scholar
  18. 18.
    Caspersson, T., Zech, L., Johansson, C., and Modest, B. J. (1970).Chromosoma 30:215–227.PubMedGoogle Scholar
  19. 19.
    Kit, S., Leung, W.-C, and Trkula, D. (1973).Arch. Biochem. Biophys. 158:503–513.PubMedGoogle Scholar
  20. 20.
    Kit, S., Dubbs, D. R., and Frearson, P. M. (1966).Int. J. Cancer 1:19.PubMedGoogle Scholar
  21. 21.
    Rotterdam Conference (1974). Second International Workshop on Human Gene Mapping; Birth Defects: Original Articles Series XI, 3 (1975), The National Foundation, New York.Google Scholar
  22. 22.
    Minna, J. D., and Coon, H. G. (1974).Nature 252:401–404.PubMedGoogle Scholar
  23. 23.
    Shows, T. B. (1972).Biochem. Genet. 7:193–204.PubMedGoogle Scholar
  24. 24.
    Van Heyningen, V., Bobrow, M., Bodmer, W. F., Povey, S., Gardiner, S. E., and Hopkinson, D. A. (1975).Cytogenet. Cell Genet. 14:353–357.PubMedGoogle Scholar
  25. 25.
    Tolley, E., and Craig, I. (1975).Biochem. Genet. 13:867–883.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • K. Willecke
    • 1
  • T. Reber
    • 1
  • R. S. Kucherlapati
    • 2
  • F. H. Ruddle
    • 3
  1. 1.Institut für Genetik der Universität zu KölnKöln 41Germany
  2. 2.Department of Biochemical SciencesPrinceton UniversityPrinceton
  3. 3.Department of BiologyYale UniversityNew Haven

Personalised recommendations