Human mitochondrial thymidine kinase is coded for by a gene on chromosome 16 of the nucleus
The expression of human mitochondrial thymidine kinase (mt TK) was investigated by polyacrylamide electrophoresis in 19 independent human—mouse somatic cell hybrids which allowed all human chromosomes to be analyzed. In 8 hybrid clones the presence of this enzymatic activity could be demonstrated. Human mt TK segregated concordantly with human adenine phosphoribosyltransferase (APRT) and human chromosome 16. Discordant segregation with all other human chromosomes was demonstrated by karyotype and isozyme analyses. These results suggest that human mt TK is coded for by a gene on chromosome 16 of the nucleus. Thus human mt TK is genetically different from human cytosol thymidine kinase which is coded for by a gene on chromosome 17. The appearance of one heteropolymer band after electrophoretic separation of human and murine mt TK supports the notion that both enzymes have dimeric structures.
KeywordsEnzymatic Activity Adenine Somatic Cell Thymidine Human Chromosome
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- 2.Kit, S., Leung, W.-C, and Trkula, D. (1974). InControl Processes in Neoplasia, (eds.) Mehlman, M. A., and Hanson, R. W., pp. 103–145, Academic Press, New York.Google Scholar
- 6.Kit, S., and Leung, W.-C. (1974).J. Cell Biol. 61:35–44.Google Scholar
- 12.Giles, R. E., and Ruddle, F. H. (1973). InTissue Culture—Methods and Applications, (eds.) Kruse, P. F., and Patterson, M. K., pp. 475–500, Academic Press, New York.Google Scholar
- 21.Rotterdam Conference (1974). Second International Workshop on Human Gene Mapping; Birth Defects: Original Articles Series XI, 3 (1975), The National Foundation, New York.Google Scholar