Somatic Cell Genetics

, Volume 8, Issue 2, pp 223–244 | Cite as

Assignment of an oligomycin-resistance locus to human chromosome 10

  • Keith A. Webster
  • Noëlynn A. Oliver
  • Douglas C. Wallace


An oligomycin-resistant variant of human fibrosarcoma HT1080 was isolated and characterized as nuclear and codominant. The mutant was stable, was not cross-resistant to respiratory inhibitors, and it contained a mitochondrial ATPase which was less sensitive to oligomycin. Hybrids formed between the human mutant and a mouse cell line expressed the resistance phenotype. By a detailed karyotypic analysis of these hybrids using trypsin-Giemsa banding it was found that resistance to oligomycin correlated with the retention of two human chromosomes 10. The hybrid lines contained only mouse mitochondrial DNA as shown by analyses of mitochondrially synthesized proteins and mitochondrial DNA. The study assigns an ATPase oligomycin-resistance locus to human chromosome 10 and suggests that mouse and human subunits can combine in a functional enzyme complex.


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Literature cited

  1. 1.
    Downie, J.A., Gibson, F., and Cox, G.B. (1979).Annu. Rev. Biochem. 48:103:131.PubMedGoogle Scholar
  2. 2.
    Futai, M., and Konazawa, H. (1980).Curr. Top. Bioenerg. 10:181–215.Google Scholar
  3. 3.
    Tzagoloff, A., Macino, G., and Selbald, W. (1979).Annu. Rev. Biochem. 48:419–441.Google Scholar
  4. 4.
    Downie, J.A., Langmans, L., Cox, G.B., Yanofsky, C., and Gibson, P. (1980).J. Bacteriol. 143:8–17.PubMedGoogle Scholar
  5. 5.
    Downie, J.A., Cox, B.G., Lengmann, C., Ash, G., Becker, M., and Gibson, F. (1981).J. Bacteriol. 145:200–210.PubMedGoogle Scholar
  6. 6.
    Gay, N.J., and Walker, J.E. (1981).Nucleic Acids Res. 9:2187–2194.PubMedGoogle Scholar
  7. 7.
    Sebald, W. (1977).Biochim. Biophys. Acta 463:1–27.PubMedGoogle Scholar
  8. 8.
    Borst, P., and Grivell, L.A. (1978).Cell 15:705–723.PubMedGoogle Scholar
  9. 9.
    Lancashire, W.E., and Maltoon. (1979).Mol. Gen. Genet. 176:255–264.PubMedGoogle Scholar
  10. 10.
    Linnane, A.W., and Nagley, P. (1978).Plasmid 1:324–345.PubMedGoogle Scholar
  11. 11.
    Saunders, G.W., Bank, G.H., Kanstermann-Kuhn, B., and Hollenberg, C.P. (1979).Mol. Gen. Genet. 175:45–52.PubMedGoogle Scholar
  12. 12.
    Breen, G.A.M., and Scheffler, I.E. (1980).J. Cell Biol. 86:723–729.PubMedGoogle Scholar
  13. 13.
    Lagarde, A.E., and Siminovitch, L. (1979).Somat. Cell Genet. 5:847–871.PubMedGoogle Scholar
  14. 14.
    Kuhns, M.C., and Eisenstadt, J.M. (1979).Somat. Cell Genet. 5:821–832.PubMedGoogle Scholar
  15. 15.
    Howel, N., and Sager, R. (1979).Somat. Cell Genet. 5:833–845.PubMedGoogle Scholar
  16. 16.
    Lichtor, T., and Getz, G.S. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:324–328.PubMedGoogle Scholar
  17. 17.
    Lichtor, T., Tung, B., and Getz, G.S. (1979).Biochemistry 18:2582–2590.PubMedGoogle Scholar
  18. 18.
    Ditta, G., Soderberg, K., Landy, F., and Scheffler I.E. (1976).Somat. Cell Genet. 2:331–344.PubMedGoogle Scholar
  19. 19.
    Breen, G.A.M., and Scheffler, I.E. (1979).Somat. Cell Genet. 5:441–451.PubMedGoogle Scholar
  20. 20.
    Soderberg, K., Mascerello, J.T., Breen, G.A.M., and Scheffler, I.E. (1979).Somat. Cell Genet. 5:225–240.PubMedGoogle Scholar
  21. 21.
    Macino, G.E., and Tzagoloff, A. (1980).Cell 20:507–517.PubMedGoogle Scholar
  22. 22.
    Macino, G.E., and Tzagoloff, A. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:131–135.PubMedGoogle Scholar
  23. 23.
    Sebald, W., Wachter, E., and Tzagloff, A. (1979).Eur. J. Biochem. 100:599–607.PubMedGoogle Scholar
  24. 24.
    Glaser, E., Norling, B., and Ernster, L. (1981).Eur. J. Biochem. 115:189–196.PubMedGoogle Scholar
  25. 25.
    Aroskar, V.A., and Avadhani, N.G. (1979).Biochem. Biophys. Res. Commun. 19:17–23.Google Scholar
  26. 26.
    Dianoux, A.C., Bof, M., and Vignais (1978).Eur. J. Biochem. 88:69–77.PubMedGoogle Scholar
  27. 27.
    deJong, L., Holtrop, M., and Kroon, A.M. (1980).Biochim. Biophys. Acta. 606:331–337.PubMedGoogle Scholar
  28. 28.
    Koch, G. (1976).J. Biol. Chem. 251:6097–6107.PubMedGoogle Scholar
  29. 29.
    Sebald, W., Sebald-Althanus, M., and Wachter, E. (1977). InGenetics and Biogenesis of Mitochondria, Vol. 2, (eds.) Bandlow, W., Scheweyen, R.J., Wolf, K., and Kandewitz, F. (W. de Gruyter and Co., Berlin), pp. 433–440.Google Scholar
  30. 30.
    Anderson, S., Bankier, A.J., Barrell, B.G., de Bruijin, M.H.C., Coulson, A.R., Drouin, J., Eperson, I.C., Nierlich, D.P., Roe, B.A., Sanger, F., Schreier, P.H., Smith, A.J.H., Staden, R., and Young, I.G. (1981).Nature 290:457–465.PubMedGoogle Scholar
  31. 31.
    Creagan, R.P., and Ruddle, F.H. (1977). InMolecular Structure of Human Chromosomes, (ed.) Yunis, J.J. (Academic Press, New York), pp. 89–142.Google Scholar
  32. 32.
    McKusick, V.A., and Ruddle, F.H. (1977).Science 196:390–405.PubMedGoogle Scholar
  33. 33.
    Rasheed, S., Nelson-Rees, W.A., Toth, E.M., Arnstein, P., and Gardner, M.B. (1974).Cancer 33:1027–1033.PubMedGoogle Scholar
  34. 34.
    Kit, S., Dubbs, D.R., and Frearson, P.M. (1966).Int. J. Cancer 1:19–30.PubMedGoogle Scholar
  35. 35.
    Klebe, R.J., Chen, J.-R., and Ruddle, F.H. (1970).J. Cell Biol. 45:74–82.PubMedGoogle Scholar
  36. 36.
    Chen, T.R. (1977).Exp. Cell Res. 104:255–262.PubMedGoogle Scholar
  37. 37.
    Wigler, M.H., and Weinstein, I.B. (1975).Biochem. Biophys. Res. Commun. 63:669–674.PubMedGoogle Scholar
  38. 38.
    Shay, J.W. (1977).Proc. Natl. Acad. Sci. U.S.A. 74:2461–2464.PubMedGoogle Scholar
  39. 39.
    Scheffler, I.E. (1975).J. Cell Physiol. 85:293–306.PubMedGoogle Scholar
  40. 40.
    Francke, U., and Oliver, N. (1978).Hum. Genet. 45:137–165.PubMedGoogle Scholar
  41. 41.
    Ronne, M., Andersen, O., and Erlander, M. (1979).Hereditas 90:195–201.PubMedGoogle Scholar
  42. 42.
    Friend, K.K., Chen, S., and Ruddle, F.H. (1976).Somat. Cell Genet. 2:183–188.PubMedGoogle Scholar
  43. 43.
    Yatscoff, R.W., and Freeman, K.B. (1977).Can. J. Biochem. 55:1064–1074.PubMedGoogle Scholar
  44. 44.
    Bodenhagen, D., and Clayton, D.A. (1974).J. Biol. Chem. 249:7991–7995.PubMedGoogle Scholar
  45. 45.
    Laemmli, U.K. (1970).Nature 227:680–685.PubMedGoogle Scholar
  46. 46.
    Giles, R.E., Stoynowski, I., and Wallace, D.C. (1980).Somat. Cell Genet. 6:543–554.PubMedGoogle Scholar
  47. 47.
    Hirt, B. (1967).J. Mol. Biol. 26:365–369.PubMedGoogle Scholar
  48. 48.
    Shipman, C., Jr., Smith, S.H., and Droch, J.C. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:1735–1757.Google Scholar
  49. 49.
    Southern, E.M. (1975).J. Mol. Biol. 98:503–517.PubMedGoogle Scholar
  50. 50.
    Rigby, P.W.J., Dieckmann, M., Rhodes, C., and Berg, P. (1977).J. Mol. Biol. 113:237–251.PubMedGoogle Scholar
  51. 51.
    Chang, A.C.Y., Lansman, R.A., Clayton, D.A., and Cohen, S.N. (1975).Cell 6:231–244.PubMedGoogle Scholar
  52. 52.
    Wallace, D.C., Bunn, C.L., and Eisenstadt, J.M. (1975).J. Cell Biol. 67:174–188.PubMedGoogle Scholar
  53. 53.
    Davidson, R.L., and Gerald, P.S. (1976).Somat. Cell Genet. 2:165–176.PubMedGoogle Scholar
  54. 54.
    O'Malley, K.A., and Davidson, R.L. (1977).Somat. Cell Genet. 3:441–448.PubMedGoogle Scholar
  55. 55.
    Kucherlapati, R.S., Hilwig, I., Geopp, A., and Ruddle, F.H. (1975).Humangenetik 27:9–14.PubMedGoogle Scholar
  56. 56.
    Croce, C.M. (1976).Proc. Natl. Acad. Sci. U.S.A. 3:441–448.Google Scholar
  57. 57.
    Weiss, M.C., and Green, H. (1967).Proc. Natl. Acad. Sci. 58:1104–1111.PubMedGoogle Scholar
  58. 58.
    Jami, J., and Grandchamp, S. (1971).Proc. Natl. Acad. Sci. U.S.A. 68:3097–3101.PubMedGoogle Scholar
  59. 59.
    DePierre, J.W., and Gurnster, L. (1977).Annu. Rev. Biochem. 46:201–262.PubMedGoogle Scholar
  60. 60.
    Brufani, M., Cellai, L., Musu, C., and Keller-Schierlein, W. (1972).Helv. Chim. Acta 55:2329–2346.PubMedGoogle Scholar
  61. 61.
    Lardy, H., Reed, P., Lin, C.H.C. (1975).Fed. Proc. 34:1707–1710.PubMedGoogle Scholar
  62. 62.
    Francke, U., and Gehring, U. (1980).Cell 22:657–664.PubMedGoogle Scholar
  63. 63.
    Wallace, D.C (1981).Mol. Cell Biol. 1:697–710.PubMedGoogle Scholar
  64. 64.
    Moore, K.H., Johnson, P.H., Chandler, L.E.W., and Grossman, L.I. (1977).Nucleic Acids Res. 4:1273–1281.PubMedGoogle Scholar
  65. 65.
    Francesco, L.D., Attardi, G., and Croce, C.M. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:4079–4083.PubMedGoogle Scholar
  66. 66.
    Macino, G.E., and Tzagoloff, A. (1979).J. Biol. Chem. 254:4617–4623.PubMedGoogle Scholar
  67. 67.
    Wiseman, A., and Attardi, G. (1979).Somat. Cell Genet. 5:241–262.PubMedGoogle Scholar
  68. 68.
    Mascarello, J.T., Soderberg, K., and Scheffler, I.E., (1980).Cytogenet. Cell Genet. 28:121–135.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1982

Authors and Affiliations

  • Keith A. Webster
    • 1
  • Noëlynn A. Oliver
    • 1
  • Douglas C. Wallace
    • 1
  1. 1.Department of Genetics, School of MedicineStanford UniversityStanford

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