Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line
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We are exploring the origin and function of amplified DNA sequences associated with double minutes (DMs) in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA from these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. From results of Northern- and Southern-blot analyses, we conclude that these cDNAs represent two distinct genes, which we have designated mdm-1and mdm-2.Using DNAs from a panel of Chinese hamster-mouse somatic cell hybrids together with in situ hybridization protocols for gene mapping studies, we have found that these DM-associated, amplified DNA sequences originate from mouse chromosome 10, region C1–C3. Sequences homologous to mdm-1and mdm-2are present in the genomes of several species examined, including that of man.
KeywordsSomatic Cell cDNA Library cDNA Clone Mapping Study Cell Hybrid
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- 2.George, D.L. (1984).Cancer Surv. 3:497–513.Google Scholar
- 11.George, D.L., and Powers, V.E. (1982). InGene Amplification, (ed.) Schimke, R. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York), pp. 199–204.Google Scholar
- 18.Cahilly, L.A., George, D.L., Daugherty, B.L., and Pestka, S. (1985).J. Int. Res. 5:391–395.Google Scholar