Somatic Cell and Molecular Genetics

, Volume 13, Issue 3, pp 235–244 | Cite as

Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line

  • Linda Cahilly-Snyder
  • Teresa Yang-Feng
  • Uta Francke
  • Donna L. George
Article

Abstract

We are exploring the origin and function of amplified DNA sequences associated with double minutes (DMs) in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA from these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. From results of Northern- and Southern-blot analyses, we conclude that these cDNAs represent two distinct genes, which we have designated mdm-1and mdm-2.Using DNAs from a panel of Chinese hamster-mouse somatic cell hybrids together with in situ hybridization protocols for gene mapping studies, we have found that these DM-associated, amplified DNA sequences originate from mouse chromosome 10, region C1–C3. Sequences homologous to mdm-1and mdm-2are present in the genomes of several species examined, including that of man.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    Bishop, J.M. (1983).Annu. Rev. Biochem. 52:301–354.PubMedGoogle Scholar
  2. 2.
    George, D.L. (1984).Cancer Surv. 3:497–513.Google Scholar
  3. 3.
    Cowell, J.K. (1982).Annu. Rev. Genet. 16:21–59.PubMedGoogle Scholar
  4. 4.
    Schwab, M., Alitalo, K., Varmus, H.E., Bishop, J.M., and George, D. (1983).Nature 303:497–501.PubMedGoogle Scholar
  5. 5.
    Brodeur, G., Seeger, R.C., Schwab, M., Varmus, H.E., and Bishop, J.M. (1984).Science 224:1121–1124.PubMedGoogle Scholar
  6. 6.
    Schwab, M., Alitalo, K., Klempnauer, K.-H., Varmus, H.E., Bishop, J.M., Gilbert, F., Brodeur, G., Goldstein, M., and Trent, J. (1983).Nature 305:245–248.PubMedGoogle Scholar
  7. 7.
    Schwab, M., Ellison, J., Busch, M., Rosenau, W., Varmus, H.E., and Bishop, J.M. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:4940–4944.PubMedGoogle Scholar
  8. 8.
    Little, C.D., Nau, M.M., Carney, D.N., Gazdar, A.F., and Minna, J.D. (1983).Nature 306:194–196.PubMedGoogle Scholar
  9. 9.
    Nau, M.M., Brooks, B.J., Battey, J., Sausville, E., Gazdar, A.F., Kirsch, I.R., McBride, O.W., Bertness, V., Hollis, G.F., and Minna, J.D. (1985).Nature 318:69–73.PubMedGoogle Scholar
  10. 10.
    Wong, A.J., Ruppert, J.M., Eggleston, J., Hamilton, S., Baylin, S.B., and Vogelstein, B. (1986).Science 233:461–464.PubMedGoogle Scholar
  11. 11.
    George, D.L., and Powers, V.E. (1982). InGene Amplification, (ed.) Schimke, R. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York), pp. 199–204.Google Scholar
  12. 12.
    Matsuya, Y., and Green, H. (1969).Science 163:397–398.PubMedGoogle Scholar
  13. 13.
    Dorman, B.P., Shimizu, N., and Ruddle, F. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:2363–2367.PubMedGoogle Scholar
  14. 14.
    George, D.L., and Francke, U. (1980).Cytogenet. Cell Genet. 28:217–226.PubMedGoogle Scholar
  15. 15.
    George, D.L., and Powers, V.E. (1981).Cell 24:117–123.PubMedGoogle Scholar
  16. 16.
    Topp, W.C. (1981).Virology 113:408–411.PubMedGoogle Scholar
  17. 17.
    Cahilly, L.A., and George, D.L. (1985).Cytogenet. Cell Genet. 39:140–144.PubMedGoogle Scholar
  18. 18.
    Cahilly, L.A., George, D.L., Daugherty, B.L., and Pestka, S. (1985).J. Int. Res. 5:391–395.Google Scholar
  19. 19.
    Aviv, H., and Leder, P. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:1408–1412.PubMedGoogle Scholar
  20. 20.
    Schwarzbauer, J., Tamkun, J.W., Lemischka, I.R., and Hynes, R.O. (1983).Cell 35:421–431.PubMedGoogle Scholar
  21. 21.
    Gorecki, M., and Rozenblatt, S. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:3686–3690.PubMedGoogle Scholar
  22. 22.
    George, D.L., Scott, A.F., Trusko, S., Glick, B., Ford, E., and Dorney, D.J. (1985).EMBO J. 4:1199–1203.PubMedGoogle Scholar
  23. 23.
    Singh, L., and Jones, K.W. (1984).Nucleic Acids Res. 12:5627–5638.PubMedGoogle Scholar
  24. 24.
    Fujii, D., Brissenden, J.E., Derynck, R., and Francke, U. (1986).Somat. Cell Mol. Genet. 12:281–288.PubMedGoogle Scholar
  25. 25.
    Feinberg, A.P., and Vogelstein, B. (1983).Anal. Biochem. 132:6–13.PubMedGoogle Scholar
  26. 26.
    Francke, U., Lalley, P.A., Moss, W., Ivy, L., and Minna, J.D. (1977).Cytogenet. Cell Genet. 19:57–84.PubMedGoogle Scholar
  27. 27.
    Francke, U., and Taggart, R.T. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:5230–5233.PubMedGoogle Scholar
  28. 28.
    Harper, M.E., Ullrich, A., and Saunders, G.F. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:4458–4460.PubMedGoogle Scholar
  29. 29.
    Lin, C.C., Draper, P.N., and DeBrackeleer, M. (1985).Cytogenet. Cell Genet. 39:269–274.PubMedGoogle Scholar
  30. 30.
    Ellis, R.W., DeFeo, D., Shih, T.Y., Gonda, M.A., Young, H.A., Tsuchida, N., Lowy, D.R., and Scolnick, E.M. (1981).Nature 292:506–511.PubMedGoogle Scholar
  31. 31.
    Sakaguchi, A.Y., Lalley, P.A., Zabel, B.U., Ellis, R.W., Scolnick, E.M., and Naylor, S.L. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:525–529.PubMedGoogle Scholar
  32. 32.
    Nesbitt, M.N., and Francke, U. (1973).Chromosoma 41:145–158.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Linda Cahilly-Snyder
    • 1
  • Teresa Yang-Feng
    • 2
  • Uta Francke
    • 2
  • Donna L. George
    • 1
    • 3
  1. 1.Department of Human GeneticsUniversity of Pennsylvania, School of MedicinePhiladelphia
  2. 2.Department of Human GeneticsYale University, School of MedicineNew Haven
  3. 3.Howard Hughes Medical InstituteUniversity of Pennsylvania, School of MedicinePhiladelphia

Personalised recommendations