Somatic Cell and Molecular Genetics

, Volume 15, Issue 3, pp 203–213 | Cite as

Mapping of prolactin and tumor necrosis factor-beta genes on human chromosome 6p using lymphoblastoid cell deletion mutants

  • Alida M. Evans
  • Jean W. Petersen
  • Gurbax S. Sekhon
  • Robert DeMars


A collection of human B lymphoblastoid cell lines (LCLs) was used to map two genetic sequences for which polymorphism had not been identified: human prolactin (PRL) and tumor necrosis factor-beta (TNFB). The LCLs have overlapping deletions on chromosome 6p produced by gamma-irradiation of LCL 721. After using two chromosome 6p sequences for which LCL 721 is heterozygous to validate our scanning denistometry (SD) method for inferring gene copy number, SD was used to map TNFBand PRL. TNFBmaps to the interval between the C4complement and HLA-Bloci within the MHC on chromosome 6p. PRLlies within the 6p21.3–6p22.2 interval distal to HLA-C.We found that LCL 721 is heterozygous for PRL DNA fragment lengths generated by HpaII but not MspI digestion, indicating that the two copies of PRLin LCL 721 are differentially methylated. This novel methylation RFLP was used to corroborate the region PRL assignment.


Tumor Necrosis Prolactin Gene Copy Fragment Length Deletion Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Literature cited

  1. 1.
    Owerbach, D., Rutter, W.J., Cooke, N.E., Martial, J.A., and Shows, T.B. (1981).Science 221:815–816.Google Scholar
  2. 2.
    Taggart, R.T., Mohandas, T.K., and Bell, G.I. (1987).Cytogenet. Cell Genet. 46:701–702.Google Scholar
  3. 3.
    Naylor, S.L., Grzeschik, K.H., and Sakaguchi, A.Y. (1984).Cytogenet. Cell Genet. 37:547–548.Google Scholar
  4. 4.
    Rose, D.P. (1986).Prev. Med. 15:99–101.PubMedGoogle Scholar
  5. 5.
    Shiu, R.P.C., Lima, G., and Iwasiow, B. (1984). InAbstracts of the 7th International Congress of Endocrinology. Excerpta Medica International Congress Series No. 652 (Elsevier, Amsterdam), p. 983.Google Scholar
  6. 6.
    Laverriere, J.N., Morin, A., Tixier-Vidal, A., Gourdji, D., and Martial, J.A. (1986).EMBO J. 2:1493–1499.Google Scholar
  7. 7.
    Lewis, U.J., Singh, R.N.P., Sigel, M.B., Frigeri, L.G., Sinha, Y.N., and Vanderlaan, W.P. (1984). InHormone Receptors in Growth and Reproduction, (ed.) Saxena, B.B. (Raven Press, New York), pp. 37–53.Google Scholar
  8. 8.
    Mittra, I. (1980).Biochem. Biophys. Res. Commun. 95:1750–1759.PubMedGoogle Scholar
  9. 9.
    Mittra, I. (1980).Biochem. Biophys. Res. Commun. 95:1760–1767.Google Scholar
  10. 10.
    Sinha, Y.N., Gilligan, T.A., and Lee, D.W. (1984).J. Clin. Endocrinol. Metab. 58:752–754.PubMedGoogle Scholar
  11. 11.
    Spies, T., Morton, C.C., Nedospasov, S.A., Fiers, W., Pious, D., and Strominger, J.L. (1986).Proc. Natl. Acad. Sci. U.S.A. 83:8699–8702.PubMedGoogle Scholar
  12. 12.
    Carroll, M.C., Katzman, P., Alicot, E.M., Koller, B.H., Geraghty, D.E., Orr, H.T., Strominger, J.L., and Spies, T. (1987).Proc. Natl. Acad. Sci. U.S.A. 84:8535–8539.PubMedGoogle Scholar
  13. 13.
    Kavathas, P., Bach, F.H., and DeMars, R. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:4251–4255.PubMedGoogle Scholar
  14. 14.
    Kavathas, P., DeMars, R., Bach, F.H., and Shaw, S. (1981).Nature 293:747–749.PubMedGoogle Scholar
  15. 15.
    Erlich, H., Lee, J.S., Petersen, J.W., Bugawan, T., and DeMars, R. (1986).Hum. Immunol. 16:205–219.PubMedGoogle Scholar
  16. 16.
    Koller, B.H., Geraghty, D., Orr, H.T., Shimizu, Y., and DeMars, R. (1987).Immunol. Res. 6:1–10.Google Scholar
  17. 17.
    Whitehead, A.S., Cohen, H.R., Chang, C.C., and DeMars, R. (1985).J Immunol. 134:641–643.PubMedGoogle Scholar
  18. 18.
    Leach, R., DeMars, R., Hasstedt, S., and White, R. (1986).Proc. Natl. Acad. Sci. U.S.A. 83:3909–3913.PubMedGoogle Scholar
  19. 19.
    Kavathas, P., and DeMars, R. (1981).Am. J. Hum. Genet. 33:935–945.PubMedGoogle Scholar
  20. 20.
    Kavathas, P., DeMars, R., and Bach, F.H. (1980).Hum. Immunol. 4:317–324.Google Scholar
  21. 21.
    Orr, H.T., Bach, F.H., Ploegh, H.L., Strominger, J.L., Kavathas, P., and DeMars, R. (1982).Nature 296:454–456.PubMedGoogle Scholar
  22. 22.
    Orr, H.T., and DeMars, R. (1983).Immunogenetics 18:489–502.PubMedGoogle Scholar
  23. 23.
    Koller, B.H., Geraghty, D.E., Shimizu, Y., DeMars, R., and Orr, H.T. (1988).J. Immunol. 141:897–904.PubMedGoogle Scholar
  24. 24.
    DeMars, R., Chang, C.C., and Rudersdorf, R.A. (1983).Hum. Immunol. 8:123–139.PubMedGoogle Scholar
  25. 25.
    Blin, N., and Stafford, D.W. (1976).Nucleic Acids Res. 3:2303–2308.PubMedGoogle Scholar
  26. 26.
    Lester, S.C., Korn, N.J., and DeMars, R. (1982).Somat. Cell Genet. 8:265–284.PubMedGoogle Scholar
  27. 27.
    Nadon, N., Korn, N., DeMars, R. (1988).Somat. Cell Mol. Genet. 14:541–552.PubMedGoogle Scholar
  28. 28.
    Nadon, N. (1987). Thesis. University of Wisconsin, Madison.Google Scholar
  29. 29.
    Nedwin, G.E., Naylor, S.L., Sakaguchi, A.Y., Smith, D., Jarrett-Nedwin, J., Pennica, D., Goeddel, D.V., and Gray, P.W. (1985).Nucleic Acids Res. 13:6361–6373.PubMedGoogle Scholar
  30. 30.
    Cooke, N.E., Coit, D., Shine, J., Baxter, J.D., and Martial, J.A. (1981).J. Biol. Chem. 256:4007–4016.PubMedGoogle Scholar
  31. 31.
    Rigby, P.W.J., Dieckmann, M., Rhodes, C., and Berg, P. (1977).J. Mol. Biol. 113:237–251.PubMedGoogle Scholar
  32. 32.
    Sokal, R.R., and Rohlf, F.J. (1969). Biometry (W.H. Freeman, San Francisco).Google Scholar
  33. 33.
    Rohlf, F.J., and Sokal, R.R. (1969). Statistical Tables (W.H. Freeman, San Francisco).Google Scholar
  34. 34.
    Holliday, R. (1987).Science 238:163–169.PubMedGoogle Scholar
  35. 35.
    Silva, A.J., and White, R. (1988).Cell 54:145–152.PubMedGoogle Scholar
  36. 36.
    Reik, W., Collick, A., Norris, M.L., Barton, S.C., and Surani, M.A. (1987).Nature 328:248–251.PubMedGoogle Scholar
  37. 37.
    Swain, J.L., Stewart, T.A., and Leder, P. (1987).Cell 50:719–727.PubMedGoogle Scholar
  38. 38.
    Truong, A.T., Duez, C., Belayew, A., Renard, A., Pictet, R., Bell, G.I., and Martial, J.A. (1984).EMBO J. 3:429–437.PubMedGoogle Scholar
  39. 39.
    Cedar, H. (1988).Cell 53:3–4.PubMedGoogle Scholar
  40. 40.
    Laverriere, J.N., Muller, M., Buisson, N., Tougard, C., Tixier-Vidal, A., Martial, J.A., and Gourdji, D. (1986).Endocrinology 118:198–206.PubMedGoogle Scholar
  41. 41.
    Olsson, H., and Ranstam, J. (1988).J. Natl Cancer Inst. 80:679–683.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Alida M. Evans
    • 1
  • Jean W. Petersen
    • 1
  • Gurbax S. Sekhon
    • 1
    • 3
  • Robert DeMars
    • 1
    • 2
  1. 1.Laboratory of GeneticsUniversity of Wisconsin-MadisonMadison
  2. 2.Department of Human OncologyUniversity of Wisconsin-MadisonMadison
  3. 3.Waisman CenterUniversity of Wisconsin-MadisonMadison

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