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Somatic Cell and Molecular Genetics

, Volume 11, Issue 5, pp 467–475 | Cite as

Selection of highly transfectable variant from mouse mastocytoma P815

  • Aline Van Pel
  • Etienne De Plaen
  • Thierry Boon
Article

Abstract

A tk cell line derived from mouse mastocytoma P815 was transfected with a plasmid carrying a thymidine kinase gene. The tk+ cells were obtained at a frequency of 10−6. From some of these tk+ transfectants it was possible to select tk cells with BrdU so that these cells could be submitted again to transfection with the thymidine kinase gene. By repeating cycles of transfection and tk+ selection followed by reverse selection with BrdU, it was possible to obtain a stable variant having a 100-fold increase in transfection efficiency. This HTR (high transfection) variant shows a high efficiency of transfection (10−4) for the neomycin-resistance gene as well as for the thymidine kinase gene.

Keywords

Thymidine Transfection Efficiency Thymidine Kinase Kinase Gene Stable Variant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Aline Van Pel
    • 1
    • 2
  • Etienne De Plaen
    • 1
    • 2
  • Thierry Boon
    • 1
    • 2
  1. 1.Ludwig Institute for Cancer ResearchBrusselsBelgium
  2. 2.Cellular Genetics UnitCatholic University of LouvainBelgium

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