Somatic Cell and Molecular Genetics

, Volume 11, Issue 1, pp 43–51 | Cite as

Effect of cell cycle position on transformation by microinjection

  • Eric A. Wong
  • Mario R. Capecchi


We have investigated the effect of cell cycle position on the efficiency of transformation by microinjection. Linear recombinant plasmids transform synchronized cells with similar frequencies following injections at all cell cycle stages, whereas supercoiled molecules show a decreased ability to generate transformants in early S phase. This inhibition is not due to an inability to transiently express a transferred gene, since cells at all stages of the cycle efficiently expressed a hamster adenine phosphoribosyltransferase gene introduced on a supercoiled plasmid. Southern transfer analyses of the cell cycle specific transformants revealed that tandem arrays of plasmids, integrated into the host chromosomes, could be generated at all cell cycle stages.


Cell Cycle Adenine Recombinant Plasmid Similar Frequency Tandem Array 
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Literature cited

  1. 1.
    Waechter, D.E., and Baserga, R. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:1106–1110.PubMedGoogle Scholar
  2. 2.
    Stein, R., Razin, A., and Cedar, H. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:3418–3422.PubMedGoogle Scholar
  3. 3.
    Cole, C.N., and Santangelo, G.M. (1983).Mol. Cell. Biol. 3:267–279.PubMedGoogle Scholar
  4. 4.
    Kratzer, P.G., Chapman, V.M., Lambert, H., Evans, R.E., and Liskay, R.M. (1983).Cell 33:37–42.PubMedGoogle Scholar
  5. 5.
    Khoury, G., and Gruss, P. (1983).Cell 33:313–314.PubMedGoogle Scholar
  6. 6.
    Chandler, V.L., Maler, B.A., and Yamamoto, K.R. (1983).Cell 33:489–499.PubMedGoogle Scholar
  7. 7.
    Lang, K.M., and Spritz, R.A. (1983).Science 220:1351–1355.PubMedGoogle Scholar
  8. 8.
    Majors, J., and Varmus, H.E. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:5866–5870.PubMedGoogle Scholar
  9. 9.
    Kruczek, I., and Doerfler, W. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:7586–7590.PubMedGoogle Scholar
  10. 10.
    Upcroft, P., Carter, B., and Kidson, C. (1980).Nucleic Acids Res. 8:2725–2736.PubMedGoogle Scholar
  11. 11.
    Chia, W., and Rigby, P.W.J. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:6638–6642.PubMedGoogle Scholar
  12. 12.
    Folger, K.R., Wong, E.A., Wahl, G., and Capecchi, M.R. (1982).Mol. Cell. Biol. 2:1372–1387.PubMedGoogle Scholar
  13. 13.
    Small, J., and Scangos, G. (1983).Science 219:174–176.PubMedGoogle Scholar
  14. 14.
    Robert de Saint Vincent, B., and Wahl, G.M. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:2002–2006.PubMedGoogle Scholar
  15. 15.
    Subramani, S., and Berg, P. (1983).Mol. Cell. Biol. 3:1040–1052.PubMedGoogle Scholar
  16. 16.
    Ruley, H.E., and Fried, M. (1983).Nature 304:181–184.PubMedGoogle Scholar
  17. 17.
    Shapira, G., Stachelek, J.L., Letsou, A., Soodak, L., and Liskay, R.M. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:4827–4831.PubMedGoogle Scholar
  18. 18.
    Pomerantz, B.J., Naujokas, M., and Hassell, J.A. (1983).Mol. Cell. Biol. 3:1680–1685.PubMedGoogle Scholar
  19. 19.
    Anderson, R.A., Kato, S., and Camerini-Otero, R.D. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:206–210.PubMedGoogle Scholar
  20. 20.
    Kucherlapati, R.S., Eves, E.M., Song, K.-Y., Morse, B.S., and Smithies, O. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:3153–3157.PubMedGoogle Scholar
  21. 21.
    Scangos, G., and Ruddle, F.H. (1981).Gene 14:1–10.PubMedGoogle Scholar
  22. 22.
    Schaffner, W. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:2163–2167.PubMedGoogle Scholar
  23. 23.
    Sandri-Goldin, R.M., Goldin, A.L., Levine, M., and Glorioso, J.C. (1981).Mol. Cell. Biol. 1:743–752.PubMedGoogle Scholar
  24. 24.
    Rigby, P.W.J. (1983).J. Gen. Virol. 64:255–266.PubMedGoogle Scholar
  25. 25.
    Graessman, A., Wolf, H., and Bornkamm, G.W. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:433–436.PubMedGoogle Scholar
  26. 26.
    Anderson, W.F., Killos, L., Sanders-Haigh, L., Kretschmer, P.J., and Diacumakos, E.G. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:5399–5403.PubMedGoogle Scholar
  27. 27.
    Capecchi, M.R. (1980).Cell 22:479–488.PubMedGoogle Scholar
  28. 28.
    Kopchick, J.J., Ju, G., Skalka, A.M., and Stacey, D.W. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:4383–4387.PubMedGoogle Scholar
  29. 29.
    Pardee, A.B., Dubrow, R., Hamlin, J.L., and Kletzien, R.F. (1978).Annu. Rev. Biochem. 47:715–750.PubMedGoogle Scholar
  30. 30.
    Sene, C., and Nicolau, C. (1981). InLiposomes, Drugs, and Immunocompetent Cells, (ed.) Nicolau, C., and Paraf, A. (Academic Press, New York), pp. 67–77.Google Scholar
  31. 31.
    Razzaque, A., Mizusawa, H., and Seidman, M.M. (1983).Proc. Natl. Acad. Sci. U.S.A. 80: 3010–3014.PubMedGoogle Scholar
  32. 32.
    Calos, M.P., Lebkowski, J.S., and Botchan, M.R. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:3015–3019.PubMedGoogle Scholar
  33. 33.
    Wake, C.T., Gudewicz, T., Porter, T., White, A., and Wilson, J.H. (1984).Mot. Cell. Biol. 4:387–398.Google Scholar
  34. 34.
    Kopchick, J.J., and Stacey, D.W. (1984).Mol. Cell. Biol. 4:240–246.PubMedGoogle Scholar
  35. 35.
    Topp, W.C. (1981)Virology 113:408–411.PubMedGoogle Scholar
  36. 36.
    Hamada, K., Isomura, K., Teranishi, K., and Watanabe, H. (1978).Mutat. Res. 54:61–72.PubMedGoogle Scholar
  37. 37.
    Lowy, I., Pellicer, A., Jackson, J.F., Sim, G.-K., Silverstein, S., and Axel, R. (1980).Cell 22:817–823.PubMedGoogle Scholar
  38. 38.
    Luciw, P.A., Bishop, J.M., Varmus, H.E., and Capecchi, M.R. (1983).Cell 33:705–716.PubMedGoogle Scholar
  39. 39.
    Huttner, K.M., Barbosa, J.A., Scangos, G., Pravtcheva, D., and Ruddle, F.H. (1981).J. Cell Biol. 91:153–160.PubMedGoogle Scholar
  40. 40.
    Sandri-Goldin, R.M., Goldin, A.L., Glorioso, J., and Levine, M. (1984).Somat. Cell Mol. Genet. 10:129–138.PubMedGoogle Scholar
  41. 41.
    Gellert, M. (1981).Annu. Rev. Biochem. 50:879–910.PubMedGoogle Scholar
  42. 42.
    Kikuchi, Y., and Nash, H. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:3760–3764.PubMedGoogle Scholar
  43. 43.
    Craig, N. and Nash, H. (1983).Cell 35:795–803.PubMedGoogle Scholar
  44. 44.
    Orr-Weaver, T.L., Szostak, J.W., and Rothstein, R.J. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:6354–6358.PubMedGoogle Scholar
  45. 45.
    Szostak, J.W., Orr-Weaver, T.L., Rothstein, R.J., and Stahl, F.W. (1983)Cell 33:25–35.PubMedGoogle Scholar
  46. 46.
    Stringer, J.R. (1982).Nature 296:363–366.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Eric A. Wong
    • 1
  • Mario R. Capecchi
    • 1
  1. 1.Department of BiologyUniversity of UtahSalt Lake City

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