Cloning and chromosomal location of human genes inducible by type I interferon
When cells are treated with interferon several new proteins are induced. We have isolated by differential screening two cDNA clones corresponding to human genes inducible by IFN-α, termed IFI-4 and IFI-54K. The accumulation of the corresponding mRNA was followed as a function of either IFN dose or of time. The IFI-4 and IFI-54K genes, as well as two previously isolated IFN-inducible genes, namely the IFI-56K and low-molecular-weight 2–5A synthetase, were localized on the human chromosomes. Using cloned probes on Southern blots of DNA from a panel of rodent-human somatic cell hybrids, we have assigned the IFI-4 gene to chromosome 1 and the gene coding for the low-molecular-weight 2–5A synthetase to chromosome 12. We also showed that the IFI-54K and IFI-56K genes, unlike most of the IFN-inducible genes, are syntenic. They are both located on chromosome 10. In addition, evidence is given for the presence of a pseudogene homologous to IFI-56K on chromosome 13.
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- 2.Content, J. (1986). InMolecular Basis of Viral Replication, (ed.) Perez-Beroff, R. (Plenum Press, New York), pp. 163–189.Google Scholar
- 23.Saunders, M.E., Gewert, D.R., Tugwell, M.E., McMahon, M., and Williams, B.R.G. (1985).EMBOJ. 4:1761–1768.Google Scholar
- 28.Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982).Molecular Cloning, A Laboratory Manual, (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).Google Scholar
- 30.Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K., and Green, M.R. (1984).Nucleic Acids Res. 12:7033–7056.Google Scholar