Somatic Cell and Molecular Genetics

, Volume 14, Issue 5, pp 415–426 | Cite as

Cloning and chromosomal location of human genes inducible by type I interferon

  • Marc G. Wathelet
  • Josiane Szpirer
  • Christine B. Nols
  • Isabelle M. Clauss
  • Lukas De Wit
  • M. Quamrul Islam
  • Göran Levan
  • Michel A. Horisberger
  • Jean Content
  • Claude Szpirer
  • Georges A. Huez


When cells are treated with interferon several new proteins are induced. We have isolated by differential screening two cDNA clones corresponding to human genes inducible by IFN-α, termed IFI-4 and IFI-54K. The accumulation of the corresponding mRNA was followed as a function of either IFN dose or of time. The IFI-4 and IFI-54K genes, as well as two previously isolated IFN-inducible genes, namely the IFI-56K and low-molecular-weight 2–5A synthetase, were localized on the human chromosomes. Using cloned probes on Southern blots of DNA from a panel of rodent-human somatic cell hybrids, we have assigned the IFI-4 gene to chromosome 1 and the gene coding for the low-molecular-weight 2–5A synthetase to chromosome 12. We also showed that the IFI-54K and IFI-56K genes, unlike most of the IFN-inducible genes, are syntenic. They are both located on chromosome 10. In addition, evidence is given for the presence of a pseudogene homologous to IFI-56K on chromosome 13.


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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Marc G. Wathelet
    • 1
  • Josiane Szpirer
    • 1
  • Christine B. Nols
    • 1
  • Isabelle M. Clauss
    • 1
  • Lukas De Wit
    • 2
  • M. Quamrul Islam
    • 3
  • Göran Levan
    • 3
  • Michel A. Horisberger
    • 4
  • Jean Content
    • 2
  • Claude Szpirer
    • 1
  • Georges A. Huez
    • 1
  1. 1.Département de Biologie MoléculaireUniversité Libre de BruxellesRhode-St-GenèseBelgium
  2. 2.Institut Pasteur du BrabantBruxellesBelgium
  3. 3.Department of GeneticsUniversity of GöteborgGöteborgSweden
  4. 4.Pharmaceutical Research, Ciba-Geigy Ltd.BasleSwitzerland

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