Somatic Cell and Molecular Genetics

, Volume 14, Issue 5, pp 415–426

Cloning and chromosomal location of human genes inducible by type I interferon

  • Marc G. Wathelet
  • Josiane Szpirer
  • Christine B. Nols
  • Isabelle M. Clauss
  • Lukas De Wit
  • M. Quamrul Islam
  • Göran Levan
  • Michel A. Horisberger
  • Jean Content
  • Claude Szpirer
  • Georges A. Huez
Article

Abstract

When cells are treated with interferon several new proteins are induced. We have isolated by differential screening two cDNA clones corresponding to human genes inducible by IFN-α, termed IFI-4 and IFI-54K. The accumulation of the corresponding mRNA was followed as a function of either IFN dose or of time. The IFI-4 and IFI-54K genes, as well as two previously isolated IFN-inducible genes, namely the IFI-56K and low-molecular-weight 2–5A synthetase, were localized on the human chromosomes. Using cloned probes on Southern blots of DNA from a panel of rodent-human somatic cell hybrids, we have assigned the IFI-4 gene to chromosome 1 and the gene coding for the low-molecular-weight 2–5A synthetase to chromosome 12. We also showed that the IFI-54K and IFI-56K genes, unlike most of the IFN-inducible genes, are syntenic. They are both located on chromosome 10. In addition, evidence is given for the presence of a pseudogene homologous to IFI-56K on chromosome 13.

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Marc G. Wathelet
    • 1
  • Josiane Szpirer
    • 1
  • Christine B. Nols
    • 1
  • Isabelle M. Clauss
    • 1
  • Lukas De Wit
    • 2
  • M. Quamrul Islam
    • 3
  • Göran Levan
    • 3
  • Michel A. Horisberger
    • 4
  • Jean Content
    • 2
  • Claude Szpirer
    • 1
  • Georges A. Huez
    • 1
  1. 1.Département de Biologie MoléculaireUniversité Libre de BruxellesRhode-St-GenèseBelgium
  2. 2.Institut Pasteur du BrabantBruxellesBelgium
  3. 3.Department of GeneticsUniversity of GöteborgGöteborgSweden
  4. 4.Pharmaceutical Research, Ciba-Geigy Ltd.BasleSwitzerland

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