Functional characterization of rat chemokine macrophage inflammatory protein-2
Expression of mRNA for the C-X-C chemokine, macrophage inflammatory protein-2 (MIP-2), is induced during acute inflammation in rat models of disease. We have characterized the phlogistic potential of rat recombinant MIP-2 (rMIP-2) protein in vitro and in vivo. Recombinant MIP-2 caused marked PMN chemotaxis in vitro, with peak chemotactic activity at 10 nM. Incubation of whole blood with rMIP-2 caused a significant loss of L-selectin and a significant increase in Mac-1 expression on the PMN surface. Under similar conditions rMIP-2 also caused a modest respiratory burst in PMNs. The intratracheal instillation of 10 and 50μg of rMIP-2 caused a significant influx of PMNs into the airspace of the lungs. Rat MIP-2 is a potent neutrophil chemotactic factor capable of causing neutrophil activation and is likely to function in PMN recruitment during acute inflammation in rat disease models.
Wolpe, S. D., B. Sherry, D. Juers, G. Davatelis, R. W. Yurt
, andA. Cerami
. 1989. Identification and characterization of macrophage inflammatory protein 2.Proc. Natl. Acad. Sci. U.S.A.
Tekamp-Olson, P., C. Gallegos, D. Bauer, J. McClain, B. Sherry, M. Fabre, S. van Deventer
, andA. Cerami
. 1990. Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues.J. Exp. Med.
Miller, M. D.
, andM. S. Krangel
. 1992. Biology and biochemistry of the chemokines: A family of chemotactic and inflammatory cytokines.Crit. Rev. Immunol.
Walz, A., F. Meloni, I. Clark-Lewis, V. von Tscharner
, andM. Baggiolini
. 1991. [Ca++
] changes and respiratory burst in human neutrophils and monocytes by NAP-1/interleukin-8, NAP-2 andgro
/MGSA.J. Leukocyte Biol.
Derynck, R., E. Balentien, J. H. Han, H. G. Thomas, D. Wen, A. K. Samantha, C. O. Zachariae, P. R. Griffin, R. Brachmann, W. L. Wong, K. Matsuchima
, andA. Rich-Mond
. 1990. Recombinant expression, biochemical characterization, and biological activities of the human MGSA/gro protein.Biochemistry
Huang, S., J. D. Paulauskis, J. J. Godleski
, andL. Kobzik
. 1992. Expression of macrophage inflammatory protein-2 and KC mRNA in pulmonary inflammation.Am. J. Pathol.
Huang, S., A. Farone, J. D. Paulauskis, J. J. Godleski
, andL. Kobzik
. 1993. Expression of neutrophil chemoattratant cytokines, KC and MIP-2 in a rat model of SO2
-induced bronchitis.Am. Rev. Respir. Dis.
Rose, C. E., C. A. Juliano, D. E. Tracey, T. Yoshimura
, andS. M. Fu
. 1994. Role of interleukin-1 in endotoxin-induced lung injury in the rat.Am. J. Respir. Cell. Mol. Biol.
Sanger, F., S. Nicklen
, andA. R. Coulson
. 1977. DNA sequencing with chain-terminating inhibitors.Proc. Natl. Acad. Sci. U.S.A.
, andG. von Jagow
. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range of 1 to 100 kDa.Anal. Biochem.
Salzman, G. C., J. M. Crowell, B. A. Martin, T. T. Trujillo, A. Romero, P. F. Mullaney
, andP. M. Labauve
. 1975. Cell classification by laser light scattering: Identification and separation of unstained leukocytes.Acta Cytol.
Freeman, G. E., C. A. Dalton
, andP. M. Brooks
. 1991. A Nycodenz gradient method for the purification of neutrophils from the peripheral blood of rats.J. Immunol. Methods
Bass, D. A., J. W. Parce, L. R. Dechatelet, P. Szejda, M. C. Seeds
, andM. Thomas
. 1983. Flow cyometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation.J. Immunol.
Tamatani, T., M. Kotani
, andM. Miyasaka
. 1991. Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies.Eur. J. Immunol.
Robinson, A. P., T. M. White
, andD. W. Mason
. 1986. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3.Immunology
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