, Volume 18, Issue 3, pp 311–322 | Cite as

Participation of serum proteins in the inflammation-primed activation of macrophages

  • Nobuto Yamamoto
  • Norman P. Willett
  • Dwight D. Lindsay
Original Articles


Inflamed lesions release degradation products of membrane lipids, lysophospholipids, and inflamed tumor tissues release alkylglycerols. Macrophages were activated by administration of lysophosphatidylcholine (lyso-Pc) or dodecylglycerol (DDG) to mice. In vitro treatment of mouse peritoneal cells (mixture of nonadherent and adherent cells) with lyso-Pc or DDG in fetal calf serum supplemented medium for 30 min, followed by 3-h cultivation of adherent cells (macrophages) alone, resulted in greatly enhanced Fc-receptor mediated phagocytic activity and Superoxide generating capacity of macrophages. The tumor lipid metabolite, DDG, is far more potent (400-fold) than lyso-Pc in terms of doses required for the maximal levels of macrophage activation. The inflammation-primed macrophage activation required a serum factor, vitamin D binding protein, as a precursor for the macrophage activating factor. Treatment of mouse peritoneal cells with 1μg lyso-Pc/ml or 50 ng DDG/ml in a serum-free 0.1 % egg albumin supplemented medium for 30 min, followed by 3-h cultivation of the treated peritoneal cells in a medium supplemented with a very small amount (0.0005–0.05%) of ammonium sulfate [20–50% saturated (NH4)2]SO4] precipitable protein fraction of FCS, resulted in greatly enhanced Superoxide generating capacity of macrophages. The ammonium sulfate precipitable fraction was found to contain vitamin D binding protein.


Adherent Cell Ammonium Sulfate Inflame Lesion Phagocytic Activity Serum Factor 
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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Nobuto Yamamoto
    • 1
    • 2
  • Norman P. Willett
    • 3
  • Dwight D. Lindsay
    • 3
  1. 1.Department of BiochemistryUSA
  2. 2.Fels Institute for Cancer Research and Molecular BiologyUSA
  3. 3.Department of Microbiology and ImmunologyTemple University School of MedicinePhiladelphia

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