Antiproliferative effects of bacillus Calmette-Guérin and interferon α2b on human bladder cancer cells in vitro
- 39 Downloads
Direct inhibitory effects of bacillus Calmette-Guérin (BCG) and interferon α2b (IFNα2b) on six human bladder carcinoma cell lines, UCRU-BL-13, UCRU-BL-17, UCRU-BL-28, 5637, T24 and J82, were studied using an in vitro proliferation assay. Effects on proliferation following exposure to BCG or IFNα2b were analysed by [3H]thymidine incorporation over 7 days. BCG had an antiproliferative effect on all bladder lines, while sensitivity to IFNα2b varied greatly, being as remarkably low as 1 U/ml for some lines. The antiproliferative effect was greatest when cells were exposed continuously to either agent, but was still evident with a limited exposure. When clinical concentrations were simulated in vitro, BCG+IFNα2b was more effective than BCG alone and as effective as a double BCG concentration. We conclude that, in addition to their immunomodulatory effects, BCG and IFNα2b directly inhibit the proliferation of human bladder cancer cells, and often at extremely low concentrations.
Key wordsBladder neoplasms Carcinoma transitional cell BCG vaccine Interferon Immunotherapy
Unable to display preview. Download preview PDF.
- 8.Ratliff TL (1994) Mechanism of action of intravesical BCG for superficial bladder cancer: In: BCG immunotherapy in bladder cancer. Cooperativa Libraria Editrice Universita di Padova. Padova, p 25Google Scholar
- 26.Jackson AM, Alexandroff AB, Fleming D, Prescott S, Chisholm GD, Carter GD, James K (1994) Bacillus Calmette-Guérin (BCG) organisms directly alter the growth of bladder tumour cells. Int J Oncol 5: 697Google Scholar
- 27.Jackson AM, James K (1994) Understanding the most successful immunotherapy for cancer. Immunologist 2: 208Google Scholar