Klinische Wochenschrift

, Volume 57, Issue 6, pp 273–277 | Cite as

Thrombin and fibrin-induced growth of fibroblasts: Role in wound repair and thrombus organization

  • J. Pohl
  • H. D. Bruhn
  • E. Christophers
Originalien

Summary

Skin fibroblast cultures were treated with various components of the blood clotting system (thrombin, fibrinogen and fibrin) during the logarithmic growth phase. Fibrin as well as thrombin showed dose-dependent growth promoting activities as revealed by cell counting and3H-thymidine uptake. No effect was seen with fibrinogen. After entrapping in polymerizing fibrin enriched by complete culture medium the cells elongated, multiplied and formed net-like interconnecting cell strands throughout the clots. Nutritional deprivation appeared as a limiting factor for eventual growth cessation. The results demonstrate active growth of fibroblasts in fibrin clots such as present in healing wounds and thrombi. The production of thrombin by the coagulation cascade does not only result in the conversion of fibrinogen to fibrin but has also a long-lasting hormone-like effect on fibroblast proliferation which is of essential importance in wound healing, thrombus organization and progression of chronic atherosclerotic lesions.

Key words

Fibrin Fibrinogen Thrombin Clot cultures Fibroblasts 

Steigerung der Fibroblastenproliferation durch Thrombin und Fibrin in vitro und ihre Rolle bei Wundheilung und Thrombusorganisation

Zusammenfassung

Fibroblastenkulturen wurden während der logarithmischen Wachstumsphase mit verschiedenen Komponenten des Gerinnungssystems (Thrombin, Fibrinogen, Fibrin) behandelt. Die Beeinflussung der proliferativen Aktivität der Fibroblasten erfolgte durch Messung der3H-TdR-Einbaurate sowie durch Zellzählung. Fibrin und Thrombin zeigten eine konzentrationsabhängige Steigerung des Zellwachstums. Fibrinogen in verschiedenen Konzentrationen war ohne Effekt. Durch Züchtung von Fibroblasten in polymerisierendem, mit Nährmedium angereichtertem Fibrin entstanden elongierte, sich im Gerinnsel netzförmig ausbreitende Zellverbände. Die Zellen vermehrten sich um das Achtfache, nach etwa 10tägiger Kulturdauer sistierte das Zellwachstum infolge nutritiver Deprivation. Die Ergebnisse zeigen, daß Fibroblasten sich in Fibringerinnseln ausbreiten und vermehren. Thrombin bewirkt neben der Fibrinogen-Fibrin-Conversion eine anhaltende Steigerung der Fibroblastenproliferation, die bei der Wundheilung, der Organisation von Thromben und der Ausbildung atheromatöser Veränderungen von Bedeutung zu sein scheint.

Schlüsselwörter

Fibrin Fibrinogen Thrombin Fibroblastenkulturen 

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • J. Pohl
    • 1
  • H. D. Bruhn
    • 2
  • E. Christophers
    • 1
  1. 1.Department of DermatologyUniversity of KielGermany
  2. 2.Department of Internal MedicineUniversity of KielGermany

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