Isoenzymes of γ-glutamyl transpeptidase in liver diseases
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Summary
A new method for the separation of isoenzymes ofγ-glutamyl-transpeptidase is described, using electrophoresis on acetate cellulose gel and a developing solution composed byγ-glutamylβ-naphthylamide, and a colored diazonium compound.
The method permits the separation of up to four different isoenzymes, which we calledγ-GT1,γ-GT2,γ-GT3,γ-GT4, the first two showing an electrophoretic migration similar to that ofα1- andα2-globulins and the other two to that ofβ-globulins.
The present technique has proved its usefulness in detecting isoenzymes in serum with values of totalγ-glutamyl-transpeptidase higher than 80 U/L.
The application of this method in 52 patients with different types of biliary obstruction and hepatocellular damage has shown that it provides new possibilities in differential diagnosis.
Key words
Enzymology γ-glutamyl-transpeptidase isoenzymes differential diagnosis of jaundiceIsoenzyme der γ-Glutamyl-Transpeptidase bei Lebererkrankungen
Zusammenfassung
Eine neue elektrophoretische Trenn-Methode für die Isoenzyme derγ-Glutamyl-Transpeptidase (γ-GT) wird beschrieben. Als Träger dient Cellulose-acetat, als Entwicklungslösung y-Glutamyl-β-Naphthylamid und eine gefärbte Diazo-Verbindung.
Mit dieser Methode lassen sich 4 Isoenzyme differenzieren (γ-GT1—γ-GT4).γ-GT1 undγ-GT2 wandern mit den α1- und α2-Globulinen,γ-GT3 und y-GT4 im Bereich derβ-Globuline.
Die beschriebene Technik erlaubt die Trennung der y-GT-Isoenzyme in Seren, deren Gesamt-γ-GT-Aktivität 80 U/l übersteigt.
Die Anwendung der Methode bei 52 Seren von Patienten mit verschiedenartigen Gallenabflußbehinderungen und Leberparenchymzellschädigungen hat gezeigt, daß sie neue differential-diagnostische Möglichkeiten eröffnet.
Schlüsselwörter
Enzymologie γ-Glutamyl-Transpeptidase Isoenzyme Differential-Diagnose des IkterusPreview
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Literature
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