Histochemistry and Cell Biology

, Volume 105, Issue 4, pp 253–260

Antigen retrieval, signal amplification and intensification in immunohistochemistry

  • Martin Werner
  • Reinhard von Wasielewski
  • Paul Komminoth
Review

Abstract

In this overview we emphasize new methods of improving immunohistochemical results in formaldehyde-fixed tissue samples. The benefit of heat-induced antigen retrieval in demasking of concealed epitopes is demonstrated. We provide guidance on the influence of heat-induced antigen retrieval in commonly applied monoclonal and polyconal antibodies. Moreover, we show the promising methods of signal amplification using biotinylated tyramine and signal intensification of diaminobenzidine reaction products by metallic ions.

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Martin Werner
    • 1
  • Reinhard von Wasielewski
    • 1
  • Paul Komminoth
    • 2
  1. 1.Institute of PathologyMedizinische Hochschule HannoverHannoverGermany
  2. 2.Division of Cell and Molecular Pathology, Department of PathologyUniversity of ZürichSwitzerland

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