Russian Chemical Bulletin

, Volume 45, Issue 12, pp 2688–2697 | Cite as

Chemo-enzymatic synthesis of the human angiogenin gene. Construction of bacterial strains, producers of human angiogenin. Elaboration of a technology for the purification and preparation of angiogenin

  • N. P. Mertvetsov
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Abstract

Chemo-enzymatic synthesis of the gene of human angiogenin, a stimulator of blood vessel growth, was performed for the first time. Cloning of the human angiogenin gene in several expressing vector systems was carried out, and several bacterial strains, producers of human angiogenin were constructed. In particular, strains, producers of “angiogenin-β-galactosidase” and “angiogenin-domains of staphylococcal protein A hybrid proteins” were obtained, as well as a bacterial strain efficiently expressing free angiogenin. Large-scale preparation of purified recombinant human angiogenin was accomplished using theE.coli JM103 pRITA16 producer strain constructed, and the biological activity of the resulting preparation was studied.

Key words

angiogenin chemo-enzymatic synthesis cloning expression producer strain hybrid proteins 
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Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • N. P. Mertvetsov
    • 1
  1. 1.Novosibirsk Institute of Bioorganic ChemistrySiberian Branch of the Russian Academy of SciencesNovosibirskRussian Federation

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