Cytokines affecting megakaryocytopoiesis in rheumatoid arthritis with thrombocytosis
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Abstract
Particular interleukins, namely interleukin (IL)-6, IL-4 and IL-1, with pro-inflammatory mediator activities have also been shown to be involved in the regulation of megakaryocytopoiesis. As rheumatoid arthritis (RA) is not uncommonly associated with reactive thrombocytosis, we investigated serum IL-la, IL-1β, IL-4 and IL-6 concentrations in RA patients with marked thrombocytosis, and compared them to the levels in RA patients with normal platelet counts and healthy volunteers. IL-1β, IL-4 and IL-6 concentrations were found to be correlated with the disease activity in both groups of RA patients, with higher serum levels of each cytokine in the thrombocythaemic group. Significant positive correlations of IL-1β and IL-4 with the platelet counts were documented only in patients with thrombocytosis. According to our results, IL-6 and IL-1β were found to be good indicators of disease activity in RA, while IL-1β and IL-4 seemed to be related more with the process of reactive thrombocytosis secondary to rheumatoid inflammation.
Key words
Rheumatoid arthritis Interleukin 1 Interleukin 4 Interleukin 6 ThrombocytosisPreview
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References
- 1.Mizel SB (1989) The interleukins. FASEB J 3:2379–2388PubMedGoogle Scholar
- 2.Gewirtz AM (1995) Megakaryocytopoiesis: the state of the art. Thromb Haemost 74:204–209PubMedGoogle Scholar
- 3.Dan K, Gomi S, Inokuchi K, Ogata K, Yamada T, Ohki I, Hasegawa S, Nomura T (1995) Effects of interleukin-1 and tumor necrosis factor on megakaryocytopoiesis: mechanisms of reactive thrombocytosis. Acta Haematol 93:67–72PubMedGoogle Scholar
- 4.Rennick D, Jackson J, Yang G, Wideman J, Lee F, Hudak S (1989) Interleukin 6 interacts with interleukin 4 and other hematopoietic growth factors to selectively enhance the growth of megakaryocytic, erythroid, myeloid, and multipotential progenitor cells. Blood 73:1828–1835PubMedGoogle Scholar
- 5.Sonoda J, Kuzuyamà Y, Tanaka S, Yokota S, Maekawa T, Clark SC, Abe T (1993) Human interleukin 4 inhibits proliferation of megakaryocyte progenitor cells in culture. Blood 81:624–630PubMedGoogle Scholar
- 6.Arend WP, Dayer JM (1990) Cytokines and cytokine inhibitors or antagonists in rheumatoid arthritis. Arthritis Rheum 33:305–315PubMedGoogle Scholar
- 7.Davis MJ, Dawes PT (1993) A disease activity index: its use in clinical trials and disease assessment in patients with rheumatoid arthritis. Semin Arthritis Rheum 23 [Suppl 1]:50–56PubMedGoogle Scholar
- 8.Madhok R, Crilly A, Watson J, Capel HA (1993) Serum interleukin 6 levels in rheumatoid arthritis: correlations with clinical and laboratory indices of disease activity. Ann Rheum Dis 52:232–234PubMedGoogle Scholar
- 9.Hollen CW, Henthorn J, Koziol JA, Burstein SA (1991) Elevated serum interleukin 6 levels in patients with reactive thrombocytosis. Br J Haematol 71:286–291Google Scholar
- 10.Firestein GS (1995) Cytokine networks in rheumatoid arthritis: implications for therapy. Agents Actions 47 [Suppl]:37–51Google Scholar
- 11.De Benedetti F, Massa M, Robbioni D, Ravelli A, Burgio GR, Martini A (1991) Correlation of serum interleukin 6 levels with joint involvement and thrombocytosis in systemic juvenile rheumatoid arthritis. Arthritis Rheum 34:1158–1163PubMedGoogle Scholar
- 12.Crilly A, Madhok R (1992) Relationship between interleukin 6 levels platelet counts in systemic juvenile rheumatoid arthritis: comment on the article by De Benedetti et al. Arthritis Rheum 35:840PubMedGoogle Scholar
- 13.Sakito S, Ueki Y, Eguchi K, Kawabe Y, Nagataki S (1995) Serum cytokines in patients with rheumatoid arthritis. Correlation of interferon gamma and tumor necrosis factor alpha with the characteristics of peripheral blood mononuclear cells. Rheumatol Int 15:31–37PubMedGoogle Scholar
- 14.Fontana A, Hengartner H, Weber E, Fehr K, Grob PJ, Cohen G (1982) Interleukin 1 activity in the synovial fluid of patients with rheumatoid arthritis. Rheumatol Int 2:49–53PubMedGoogle Scholar
- 15.Vellenga E, Dokter W, Halie R (1993) Interleukin 4 and its receptor; modulating effects on immature and mature hematopoietic cells. Leukemia 7:1131–1141PubMedGoogle Scholar
- 16.Smeland EB, Blomhoff HK, Funderud S, Shalaby MR, Espevik T (1989) Interleukin 4 induces selective production of interleukin 6 from normal human B lymphocytes. J Exp Med 170:1463–1468PubMedGoogle Scholar
- 17.Suzuki H, Sugiyama E, Tunru IS, Yamashita N, Matsuno H, Hamazaki T, Kobayashi M (1993) Suppressive effect of interleukin 4 (IL-4) on IL-6 production by adherent rheumatoid synovial cells. Clin Immunol Immunopathol 66:67–72PubMedGoogle Scholar
- 18.Peschel C, Paul WE, Ohara J, Green I (1987) Effects of B cell stimulatory factor- 1/interleukin-4 on hematopoietic progenitor cells. Blood 70:254–259PubMedGoogle Scholar
- 19.Miossec P, Naviliat M, D'Angeac AD, Sany J, Banchereau J (1990) Low levels of interleukin 4 and high levels of transforming growth factor β in rheumatoid arthritis. Arthritis Rheum 33:1180–1187PubMedGoogle Scholar
- 20.Luqmani R, Sheeran T, Robinson M, Richardson K, Winkles J, Emery P (1994) Systemic cytokine measurements: their role in monitoring the response to therapy in patients with rheumatoid arthritis. Clin Exp Rheumatol 12:503–508PubMedGoogle Scholar