Relationship between interleukin-8 and neutrophil adhesion molecules in rheumatoid arthritis
To evaluate the role of interleukin-8 (IL-8) on the activation of neutrophils in rheumatoid arthritis (RA), we measured IL-8 and the adhesion molecules, L-selectin (CD62L), CD11b and CD18, on neutrophils in paired peripheral blood and synovial fluid of RA patients. Synovial fluid IL-8 levels were significantly increased compared to peripheral blood. L-selectin was split off and CD 11b and CD18 were upregulated on neutrophils in the synovial fluid. A positive correlation occurred between the IL-8 concentration and CD18 or CD11b densities on neutrophils in the synovial fluid (r=0.75,P <0.005 andr=0.60,P < 0.05, respectively). Peripheral blood neutrophils of the patients were desensitised with IL-8 in vivo, as shown by the significantly lower L-selectin shedding after in vitro IL-8 stimulation: 1.6 times decrease for patients vs 3.2 for controls (P < 0.05). In conclusion, these results add further evidence for the role of IL-8 in the activation of neutrophils in RA.
KeywordsInterleukin-8 CD 11b CD 18 L-selectin rheumatoid arthritis
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- 1.Endo H, Akahoshi T, Takagishi K, Kashiwazaki S, Matsushima K (1991) Elevation of interleukin-8 (IL-8) levels in joint fluids of patients with rheumatoid arthritis and the induction by IL-8 of leukocyte infiltration and synovitis in rabbit joints. Lymphokine Cytokine Res 10:245–252PubMedGoogle Scholar
- 10.Mertens AC, De Clerck LS, Moens MM, Bridts CH, Stevens WJ (1993) Lymphocyte activation status, expression of adhesion molecules and adhesion to human endothelium in rheumatoid arthritis — relationship to disease activity. Res Immunol 144:101–108Google Scholar