Plant and Soil

, Volume 162, Issue 1, pp 31–37 | Cite as

Labelling of animal manure nitrogen with15N

  • P. Sørensen
  • E. S. Jensen
  • N. E. Nielsen
Article

Abstract

A sheep was fed on15N-labelled ryegrass hay during a period of 9 days in order to obtain15N-labelled manure. After 9 days of feeding, the total N in faeces contained 3.70 atom %15N excess, which was equivalent to 82% of the15N enrichment of the hay N. The easily-decomposable fraction of the faecal N was less labelled (2.89 atom %15N excess) than the slowly-decomposable fraction. The15N enrichment of mineralized faecal N did not change significantly during 32 weeks of incubation in sand. About 25% of the faecal N was water-soluble. This N had a higher15N enrichment than the total faecal N, indicating that a part of the water-soluble N was indigestible feed N. The faeces contained only small amounts of NH4+-N, which had a15N enrichment similar to the15N enrichment of N mineralized during incubation in sand. It is suggested that the labelled faecal N obtained after a few days of feeding on labelled feed could be divided in two N pools: A decomposable N fraction (about 60%) with a15N enrichment similar to the enrichment of N mineralized in sand (2.89 ± 0.09 atom %15N excess), and a very slowly-decomposable N fraction (about 40%) with a15N enrichment similar to that of the feed (4.52 atom %15N excess).

Key words

homogeneity of labelling mineralization mobile nylon bag ryegrass hay sheep manure water-soluble N 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ayoma M 1985 Properties of fine and water-soluble fractions of several composts. 1. Micromorphology, elemental composition and nitrogen mineralization of fractions. Soil Sci. Plant Nutr. 31, 189–198.Google Scholar
  2. Castellanos J Z and Pratt P F 1981 Mineralization of manure nitrogen-correlation with laboratory indexes. Soil Sci. Soc. Am. J. 45, 354–357.Google Scholar
  3. Danfær A 1990 A Dynamic Model of Nutrient Digestion and Metabolism in Lactating Dairy Cows. 671 Report from the National Institute of Animal Science, Denmark. Landhusholdningsselskabets Forlag, Frederiksberg, Denmark. 511 p.Google Scholar
  4. Herbst F, Aziz F and Garz J 1987 Stickstoffumsetzungen im Boden nach Zugabe von15N-markierter Gülle. Arch. Acker- Pflanzenbau Bodenkd. 31, 169–175.Google Scholar
  5. Hvelplund T 1985 Digestibility of rumen microbial protein and undegraded protein estimated in the small intestine of sheep and by in sacco procedure. Acta Agric. Scand., Suppl. 25, 132–144.Google Scholar
  6. Jensen E S 1991 Evaluation of automated analysis of15N and total N in plant material and soil. Plant and Soil 133, 83–92.Google Scholar
  7. Kamphake L J, Hannah S A and Cohen J M 1967 Automated analysis for nitrate by hydrazine reduction. Water Res. 1, 205–216.Google Scholar
  8. Keeney D R and Nelson D W 1982 Nitrogen — inorganic forms.In Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties. Agronomy Monographs No 9, 2nd edn. Ed. AL Page. pp 643–698. ASA-SSSA, Madison.Google Scholar
  9. Kirchmann H 1985 Losses, plant uptake and utilisation of manure nitrogen during a production cycle. Acta Agric. Scand. Suppl. 24, 1–77.Google Scholar
  10. Nolan J V 1975 Quantitative models of nitrogen metabolism in sheep.In Digestion and Metabolism in the Ruminant. Proceedings of the IV International Symposium on Ruminant Physiology, Sydney, Australia. Eds IW McDonald and ACI Warner. pp 416–431. The University of New England Publishing Unit, Armidale.Google Scholar
  11. Peschke H 1982 Gezielte15N-Anreicherung von organischen Düngemitteln für Versuchszwecke. 2. Mitt.: Herstellung von15N-markierter Gülle. Isotopenpraxis 18, 104–106.Google Scholar
  12. Rauhe K and Bornhak H 1970 Die Wirkung von15N-markiertem Rinderkot mit verschiedenen Zusätzen im Feldversuch unter besonderer Berücksichtigung der Reproduction der organischen Substanz im Boden. Albrecht-Thaer-Archiv 14, 937–948.Google Scholar
  13. Rauhe K, Fichtner E, Fichtner F Knappe E and Drauschke W 1973 Quantifizierung der Wirkung organischer und mineralischer Stickstoffdünger auf Pflanze und Boden unter besonderer Berücksichtigung15N-markierter tierischer Exkremente. Arch. Acker-Pflanzenbau Bodenkd. 17, 907–916.Google Scholar
  14. SAS 1989 SAS Institute Inc., User's Guide, Version 6, Fourth Edition, Vol. 2, Cary, North Carolina.Google Scholar
  15. Sørensen P and Jensen E S 1991 Sequential diffusion of ammonium and nitrate from soil extracts to a polytetrafluoroethylene trap for15N determination. Anal. Chim. Acta 252, 201–203.Google Scholar
  16. Sørensen P, Jensen E S and Nielsen N E 1994 The fate of15N-labelled organic nitrogen in sheep manure applied to soils of different texture under field conditions. Plant and Soil 162, 39–47.Google Scholar
  17. Technicon 1974 Technichon Auto Analyzer II Method No. 345–74 W. Ammonia in Water and Waste Water. Technichon Industrial Systems, Tarrytown, New York.Google Scholar
  18. Thompson R B, Ryden J C and Lockyer DR 1987 Fate of nitrogen in cattle slurry following surface application or injection to grassland. J. Soil Sci. 38, 689–700.Google Scholar
  19. Van Faassen H G and Van Dijk H 1987 Manure as a source of nitrogen and phosphorus in soils.In Animal Manure on Grassland and Fodder Crops. Eds HG van der Meer, RJ Unwin, TA van Dijk and GC Ennik. pp 27–45. Martinus Nijhoff Publ, Dordrecht.Google Scholar
  20. Varvikko T and Vanhatalo A 1990 The effect of differing types of cloth and of contamination by non-feed nitrogen on intestinal digestion estimates using porous synthetic-fibre bags in a cow. British J. Nutr. 63, 221–229.Google Scholar
  21. Vilsmeyer K and Gutser R 1990 N-Ausnutzung nach Mineral- und Gülledüngung (15N) im Weihenstephaner Lysimeter. Proceedings of the 101st VDLUFA Congress. pp 175–180. VDLUFA-Schriftenreihe, 30, VDLUFA-Verlag, Darmstadt, Germany.Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • P. Sørensen
    • 1
  • E. S. Jensen
    • 1
  • N. E. Nielsen
    • 2
  1. 1.Plant Biology Section, Environmental Science and Technology DepartmentRisø National LaboratoryRoskildeDenmark
  2. 2.Section of Soil, Water and Plant Nutrition, Department of Agricultural SciencesThe Royal Veterinary and Agricultural UniversityFrederiksberg CDenmark

Personalised recommendations