Radiation and Environmental Biophysics

, Volume 23, Issue 4, pp 235–243 | Cite as

Hydrogen peroxide and hydroxyl radical formation by methylene blue in the presence of ascorbic acid

  • G. R. Buettner
  • Th. P. Doherty
  • Th. D. Bannister


Using ESR we have demonstrated the formation of the ascorbate free radical from sodium ascorbate, methylene blue and light. In oxygen uptake experiments we have observed the production of hydrogen peroxide while spin trapping experiments have revealed the iron catalyzed production of the hydroxyl free radical in this system. The presence of this highly reactive radical suggests that it could be the radical that initiates free radical damage in this photodynamic system.


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • G. R. Buettner
  • Th. P. Doherty
  • Th. D. Bannister

There are no affiliations available

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