Journal of tissue culture methods

, Volume 14, Issue 2, pp 51–57 | Cite as

Cell culture for three-dimensional modeling in rotating-wall vessels: An application of simulated microgravity

  • Ray P. Schwarz
  • Thomas J. Goodwin
  • David A. Wolf
Contributed Papers


High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 × 107 cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.

Key words

three-dimensional cell culture microcarrier cell culture BHK cells high-density cell culture rotating-wall vessel bioreactor 


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Copyright information

© Tissue Culture Association 1992

Authors and Affiliations

  • Ray P. Schwarz
    • 1
  • Thomas J. Goodwin
    • 1
  • David A. Wolf
    • 2
  1. 1.KRUG Life SciencesHouston
  2. 2.NASA, Johnson Space CenterHouston

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