Protoplasma

, Volume 182, Issue 3–4, pp 126–148

Biochemistry and structure of the glycan secreted by desiccation-tolerantNostoc commune (Cyanobacteria)

  • Donna R. Hill
  • A. Peat
  • M. Potts
Article

Summary

Filaments of the desiccation-tolerant cyanobacteriumNostoc commune are embedded within, and distributed throughout, a dense glycan sheath. Analysis of the glycan of field materials and of pure cultures ofN. commune DRH 1 through light and electron microscopy, immunogold labelling and staining with dyes, revealed changes in the pattern of differentiation in glycan micro-structure, as well as localized shifts in pH, upon rehydration of desiccated field material. A Ca/Si rich external (pellicular) layer of the glycan acts as a physical barrier to epiphytic bacteria on the surface ofN. commune colonies. A purified fraction (>12 kDa) of an aqueous extract of the glycan from desiccated field material contained glucose, N-acetylglucosamine, glucosamine, mannose, and galactosamine with ratios of 3.1∶1.4∶1∶0.1∶0.06, respectively. Lipid soluble extracts ofN. commune contained trehalose and sucrose and the levels of both became undetectable following cell rehydration. Intracellular cyanobacterial trehalase was identified using immunoblotting and its synthesis was detected upon rehydration of desiccated field cultures. Elemental analysis of glycan extracts showed a flux in the concentrations of salts in the glycan matrix following rehydration of desiccated colonies. Water-stress proteins (Wsp; most abundant proteins in glycan), a water soluble UV-A/B-absorbing pigment, the lipid-soluble UV-protective pigment scytonemin (in both its oxidized and reduced forms), as well as two unidentified cyanobacterial glycoproteins (75 kDa and 110 kDa), were found within the glycan matrix. An unidentified 68 kDa protein, the second most abundant protein in aqueous extracts of the glycan, was isolated and its N-terminal sequence was determined as AFIFGTISPNNLSGTSGNSGIVGSA. Gene bank searches with this sequence identified significant homologies (35–45%) with various carbohydrate-modifying enzymes. The role of the glycan in the desiccation tolerance ofN. commune is discussed with respect to structure/function relationships.

Keywords

UV-absorbing pigments Protein secretion Capsule Glycoproteins Trehalose 

Abbreviations

EPS

extracellular polysaccharides

Wsp

water-stress protein

SEM

scanning electron microscopy

TEM

transmission electron microscopy

EDX

energy dispersive X-ray analysis

FPLC

fast performance liquid chromatography

SDS-PAGE

sodium dodecylsulfate polyacrylamide gel electrophoresis

TLC

thin layer chromatography

UV

ultra-violet radiation

UTEX

University of Texas Culture Collection

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Donna R. Hill
    • 1
  • A. Peat
    • 1
  • M. Potts
    • 1
  1. 1.Department of BiochemistryVirginia Polytechnic Institute and State UniversityBlacksburgUSA
  2. 2.PhylaroqueFrance

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